Preparation of Two Series of Oligo-guluronic Acids from Sodium Alginate by Acid Hydrolysis and Enzymatic Degradation

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概要

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• The objective of this study was to prepare two series of authentic oligo-guluronic acids from sodium alginate. Oligo-guluronic acids (DP=1-9) were prepared from an acid hydrolysate of poly-guluronic acid by successive chromatographies of Bio-Gel P-6 and Q Sepharose Fast Flow. Oligo-guluronic acids having 4-deoxy-L-erythro-hex-4-enopyranosyluronic acid residues at the non-reducing end (DP=2-7) were prepared from the enzymatic degradation products of the poly-guluronic acid in the same manner. Each of the isolated oligo-guluronic acids gave a single band on fluorophore-assisted carbohydrate electrophoresis. These results suggest that successive chromatographies used in this study are well suited for the preparation of alginate-derived oligouronic acids.

• 社団法人日本農芸化学会の論文
• 1996-09-23

著者

• Murakami K

  Research And Development Department Kibun Food Chemifa Co. Ltd.

• Kusakabe Isao

  Institute of Applied Biochemistry, University of Tsukuba

• YOSHIDA Shigeki

  Institute of Applied Biochemistry, University of Tsukuba

• MURATA Katsumi

  Research Development Section, Kibun Food Chemifa Co., Ltd.

• SHIMOKAWA Tomoko

  Institute of Applied Biochemistry, University of Tsukuba

• TAKEUCHI Toshio

  Research and Development Department, Kibun Food Chemifa Co., Ltd.

• ISHII Tadashi

  Forestry and Forest Products Research Institute

• Yoshida S

  Plant Science Center Riken Institute

• Murata K

  Aoba Kasei Co. Ltd.

• Yoshida S

  Laboratory Of Cancer Cell Biology Research Institute For Disease Mechanism And Control Nagoya Univer

• Murakami K

  Univ. Tsukuba Ibaraki Jpn

• Murata K

  Kobe Oji Zoo Kobe Jpn

• Takeuchi Toshio

  Research And Development Department Kibun Food Chemifa Co. Ltd.

• Yoshida Shigeki

  Inst. Applied Biochem. Tsukuba Univ.

• Ishii T

  Sumitomo Chemical Co. Ltd. Takarazuka Jpn

• SHIMOKAWA Tomoko

  Forestry and Forest Products Res. Inst.

• Kusakabe I

  Univ. Tsukuba Tsukuba Jpn

• Kusakabe Isao

  Institute Of Applied Biochemistry University Of Tsukuba

• Ishii Tadashi

  Forestry And Forest Products Res. Inst. Ibaraki Jpn

• Yoshida Shigeki

  Institute Of Applied Biochemistry University Of Tsukuba

• Ishii Tadashi

  Forest Products. Res. Inst. Ibaraki

• Ishii Tadashi

  Forest And Forest Products Research Institute

• MURATA Katsumi

  Research and Development Department, Kibun Food Chemifa Co., Ltd.

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