Extracellular Carbohydrate Esterase from the Basidiomycete Coprinopsis cinerea Released Ferulic and Acetic Acids from Xylan
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概要
- 論文の詳細を見る
cDNA encoding an extracellular carbohydrate esterase (CcEst1) was cloned from the basidiomycete Coprinopsis cinerea. The recombinant CcEst1 expressed in Pichia pastoris acted on p-nitrophenyl acetate, α-naphthyl acetate, and methyl hydroxycinnamic acids, except for methyl sinapic acid. The enzyme released ferulic and acetic acids from wheat arabinoxylan and acetylated xylan respectively. Activity increased on the addition of endo-β-1,4-xylanase.
- 社団法人 日本農芸化学会の論文
- 2010-08-23
著者
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Kaneko Satoshi
Food Biotechnology Div. National Food Res. Inst.
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YOSHIDA Makoto
Faculty of Fisheries, Nagasaki University
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Hashimoto Kohsuke
Faculty Of Agriculture Tokyo University Of Agriculture And Technology
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Yoshida Makoto
Faculty Of Agriculture Tokyo University Of Agriculture And Technology
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Hashimoto Kohsuke
Fac. Of Agriculture Tokyo Univ. Of Agriculture And Technol.
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