Induction of Cell Division Synchrony and Variation of Cytokinin Contents through the Cell Cycle in Tobacco Cultued Cells
スポンサーリンク
概要
- 論文の詳細を見る
Cell division synchrony was induced in tobacco (Nicotiana tabacum) cultured cells by several treatments. Very high synchrony throughout two cell cycles was induced by aphidicolin treatment (inhibitor of DNA polymerase α, 10μg/ml) and by treatment with low temperature (4℃) and hydroxyurea (50μg/ml). The mitotic index reached its maximum (52% and 40% in aphidicolin and hydroxyurea treatments, respectively) at 11 h after removal of the added chemical. During the treatments, the cells were arrested in the G_1/S phase of the cell cycle. In the aphidicolin-induced system, incorporation of <14>^C-thymidine confirmed that DNA synthesis was started immediately after removal of the chemical. The aphidicolin-induced synchronous cells were used to study the contents of butanol-soluble cytokinins during the cell cycle. Cytokinin contents increased conspicuously at the G_2/M boundary.
- 日本植物生理学会の論文
著者
-
SYONO Kunihiko
Department of Pure and Applied Sciences, The University of Tokyo
-
Nishinari Noriko
Department Of Pure And Applied Sciences College Of Arts And Sciences University Of Tokyo:(present)de
-
Syono K
Univ. Tokyo Tokyo Jpn
-
Syono Kunihiko
Department Of Life Science Graduate School Of Arts And Sciences University Of Tokyo:(present Address
関連論文
- Occurrence of Brassinolide and Castasterone in Crown Gall Cells of Catharanthus roseus(Biological Chemistry)
- Different Expression Patterns of the Promoters of the NgrolB and NgrolC Genes during the Development of Tobacco Genetic Tumors
- The Regulatory Functions of the rolB and rolC Genes of Agrobacterium rhizogenes are Conserved in the Homologous Genes (Ngrol) of Nicotiana glauca in Tobacco Genetic Tumors : GENE STRUCTURE AND EXPRESSION : GENE STRUCTURE AND EXPRESSION
- Physiological studies of secondary metabolites in Curcuma species : production in vitro and effects on other organisms
- Detection of the IAA Biosynthetic Pathway from Tryptophan via Indole-3-Acetamide in Bradyrhizobium spp
- Function of Ngrol Genes in the Evolution of Nicotiana glauca : Conservation of the Function of NgORF13 and NgORF14 after Ancient Infection by an Agrobacterium rhizogenes-Like Ancestor
- Endogenous Levels of Gibberellins, IAA and Cytokinins in Catharanthus Crown Gall Tissues of Different Tumor Types
- Morphological Observations and Qualitative and Quantitative Studies of Auxins after Induction of Tobacco Genetic Tumor
- Endogenous Levels of Gibberellins, IAA and Cytokinins in Tobacco Crown Gall Tissues of Different Morphologies
- The Excessive Production of Indole-3-Acetic Acid and Its Significance in Studies of the Biosynthesis of This Regulator of Plant Growth and Development
- Efficient Conversion of L-Tryptophan to Indole-3-Acetic Acid and/or Tryptophol by Some Species of Rhizoctonia
- Detection OFIAA BIOSYNTHESIS IN SOME SPECIES OF RHIZOCTONIA
- PCP Award PRESENCE OF A PATHWAY FOR THE BIOSYNTHESIS OF AUXIN VIA INDOLE-3-ACETAMIDE IN TRIFOLIATA ORANGE.
- INVOLVEMENT OF _L-TRYPTOPHANAMINOTRANSFERASE IN INDOLE-3-ACETIC ACID BIOSYNTHESIS INENTEROBACTER CLOACAE
- Isolation and Characterization of a cDNA That Encodes a Novel Proteinase Inhibitor I from a Tobacco Genetic Tumor
- Presence of a Pathway for the Biosynthesis of Auxin via Indole-3-Acetamide in Trifoliata Orange
- Changes in Morphology, Levels of Endogenous IAA and Protein Composition in Relation to the Development of Tobacco Genetic Tumor Induced in the Dark
- The Presence of an Enzyme that Converts Indole-3-acetamide into IAA in Wild and Cultivated Rice
- Induction of Cell Division Synchrony and Variation of Cytokinin Contents through the Cell Cycle in Tobacco Cultued Cells
- Tumorization-Redifferentiation System of Tobacco Genetic Tumor
- The Identification of Indole-3-Acetic Acid and Indole-3-Acetamide in the Hypocotyls of Japanese Cherry
- Two Ineffective-Nodulating Mutants of Lotus japonicus-Different Phenotypes Caused by the Blockage of Endocytotic Bacterial Release and Nodule Maturation-
- Biosynthesis of cytokinins by tobacco cell cultures
- Synergistic Function of rolB, rolC, ORF13 and ORF14 of TL-DNA of Agrobacterium rhizogens in Hairy Root Induction in Nicotiana tabacum
- Photosynthesis-Dependent Volume Regulation in Guard Cell Protoplasts from Vicia faba L.
- Effect of CO_2 on Volume Change of Guard Cell Protoplast from Vicia faba L.
- A exopolysaccharide-defective mutant of Rhizobium loti eliciting abnormal nodulation
- Rhizobium Attachment and Curling in Asparagus, Rice and Oat Plants
- Hair Curling Induced in Heterologous Legumes and Monocots by Flavonoid-Treated Rhizobia
- Genetic Analysis of the Effects of Polar Auxin Transport Inhibitors on Root Growth in Arabidopsis thaliana
- Identification of cytokinins associated with mitosis in synchronously cultured tobacco cells
- Tobacco Genetic Tumors
- Isolation of Rhizobium leguminosarum biovar viciae Variants with Indole-3-acetamide Hydrolase Activity
- Cultivation of Rice Protoplasts and Their Transformation Mediated by Agrobacterium Spheroplasts
- Physiological Role of Leghaemoglobin Heterogeneity in Pea Root Nodule Development
- Effects of Leghaemoglobin Components on Nitrogen Fixation and Oxygen Consumption
- Hormonal Control of Elongation of Tobacco Cells Derived from Protoplasts
- Correlation between induction of auxin-nonrequiring tobacco calluses and increase inhibitor (s) of IAA-destruction activity