Identification of cytokinins associated with mitosis in synchronously cultured tobacco cells

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概要

• 論文の詳細を見る

• The amount of endogenous cytokinins in the butanol-soluble fraction increased about 5 times during the mitosis of synchronously dividing cultured tobacco cells (Nicotiana tabacum cv. Xanthi). The free cytokinins were identified as trans-zeatin and zeatin ribonucleoside by paper and thin layer chromatography and by high-performance liquid chromatography combined with mass spectrometry and bioassay. The water-soluble fraction contained zeatin ribonucleotide, isopentenyladenosine monophosphate, phosphoglucosylzeatin and phosphoglucosylzeatin ribonucleoside. Changes in the cytokinins in the water-soluble and transter RNA fractions during the cell cycle did not support the hypothesis that they might be the source of the butanol-soluble cytokinins which increased during mitosis. These seem to be synthesized de novo during mitosis.

• 日本植物生理学会の論文

著者

• Nishinari Noriko

  Department Of Pure And Applied Sciences College Of Arts And Sciences University Of Tokyo:(present)de

• Nishinari Noriko

  Department Of Pure And Applied Sciences Qollege Of General Education University Of Tokyo

• Shono Kunihiko

  Department Of Pure And Applied Sciences Qollege Of General Education University Of Tokyo

関連論文

• Induction of Cell Division Synchrony and Variation of Cytokinin Contents through the Cell Cycle in Tobacco Cultued Cells
• Biosynthesis of cytokinins by tobacco cell cultures
• Identification of cytokinins associated with mitosis in synchronously cultured tobacco cells

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