Changes in Tubulin Protein Expression in Guard Cells of Vicia faba L.Accompanied with Dynamic Organization of Microtubules during the Diurnal Cycle
スポンサーリンク
概要
- 論文の詳細を見る
We previously reported that the organization of microtubules (MTs) in guard cells of Vicia faba L. shows dynamic diurnal changes [Fukuda et al. (1998) Plant Cell Physiol. 39:80]. Here, we report a method to directly extract total proteins from guard cells to investigate the biochemical changes in guard cells of Vicia faba L. during the diurnal cycle. Electrophoretic profiles of total proteins of guard cells showed distinct patterns with the time of extraction. Immunoblot analysis also demonstrated changes in α-tubulin and β-tubulin contents with the diurnal cycle. Both tubulins were abundant at 6:00h and 12:00h but were almost undetectable at 24:00h. Although treatment with either actinomycin D or cycloheximide at 18:00h inhibited neither radial organization of cortical MTs norstomatal opening, that at 6:00h inhibited both. These results suggest that the dynamic diurnal changes in the organization of MTs in guard cells and stomatal movement of Vicia faba L. may be, at least partly, regulated by de novo synthesis and decomposition of tubulin molecules in guard cells.
- 日本植物生理学会の論文
著者
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HASEZAWA Seiichiro
Department of Integrated Biosciences, Graduate School of Frontier Sciences, University of Tokyo
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Nakajima N
National Inst. For Environmental Studies Ibaraki Jpn
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Nakajima Nobuyoshi
Biotechnology Research Team National Institute For Environmental Studies
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KONDO Noriaki
Department of Biological Siences, Graduate School of Sience, The University of Tokyo
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馳澤 盛一郎
東大院新領域 先端生命科学
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Kondo Noriaki
Department Of Biological Sciences Graduate School Of Science The University Of Tokyo
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Kondo Noriaki
Department Of Biological Siences Graduate School Of Sience The University Of Tokyo
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Nakajima Nobuyoshi
Biotech Inst. Fac. Bioresource Sci. Akita Pref. Univ.
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Fukuda Megumi
Grad. Sch. Sci. Univ.
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Fukuda Megumi
Department Of Dental Radiology School Of Dentistry Health Sciences University Of Hokkaido
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Fukuda Megumi
Department Of Biological Sciences University Of Tokyo
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Hasezawa Seiichiro
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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Kondo Noriaki
Grad. Sch. Sci. Univ.
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Nakajima N
National Inst. Environmental Studies Ibaraki Jpn
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