Primary Structure and Properties of Glutathione Reductase from Arabidopsis thaliana
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概要
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A cDNA encoding glutathione reductase (EC 1.6.4.2) from Arabidopsis thaliana was cloned by immunoscreening. The amino acid sequence deduced from the nucleotide sequence agrees with the N-terminal amino acid sequence of the major isozyme (GR II) purified from leaves of A. thaliana. The predicted polypeptide comprises an N-terminal leader sequence of 74 amino acids, which has features of chloroplast-targeting peptides, and a mature polypeptide of 491 residues with a molecular mass of 52.7 kDa, which shows homology with glutathione reductases from other species. The K_m for GSSG was 44μM and that for NADPH was 5.0μM for GR II at 25℃. The pH optimum for GR II was 7.5 to 8.0. The native molecular mass of GR II was 110 kDa, indicating that GR II is a homodimer. GR II had an isoelectric point of 4.8. The cDNA hybridizes with a 2.1-kb poly(A)^+RNA from leaves of A. thaliana. Genomic Southern analysis indicates that the gene corresponding to the cDNA is likely a single-copy gene.
- 日本植物生理学会の論文
著者
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Saji Hikaru
Regional Environment Division National Institute For Environmental Studies
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KUBO Akihiro
Environmental Biology Division
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Kubo A
National Inst. For Environmental Studies Ibaraki Jpn
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Sano Toshio
Department Of Biological Sciences Graduate School Of Science University Of Tokyo
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Kondo Noriaki
Department Of Biological Sciences Graduate School Of Science The University Of Tokyo
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Kondo Noriaki
Department Of Biological Siences Graduate School Of Sience The University Of Tokyo
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KONDO Noriaki
Regional Environment Division, National Institute for Environmental Studies
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TANAKA Kiyoshi
Environmental Biology Division, National Institute for Environmental Studies
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Sano Toshio
The Laboratory Of Veterinary Surgery Graduate School Of Agricultural And Life Sciences The Universit
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Sano Tomoko
Graduate School Of Environmental Science University Of Tsukuba
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TANAKA Kunisuke
College of Agriculture, Kyoto Prefectural University
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Kondo Noriaki
Grad. Sch. Sci. Univ.
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Tanaka Kiyoshi
Environmental Biology Division National Institute For Environmental Studies
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Kondo Noriaki
Regional And Community Environment Division National Lnstitute For Environmental Studies
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Kubo A
Laboratory Of Genetic Engineering Faculty Of Agriculture Kyoto Prefectural University
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