Bacteroids Isolated from Ineffective Nodules of Pisum sativum Mutant E135 (sym13) Lack Nitrogenase Activity but Contain the Two Protein Components of Nitrogenase
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概要
- 論文の詳細を見る
Pea mutant E135 (sym13) forms ineffective (Fix^-) nodules that lack nitrogen fixing activity. To determine the developmental step blocked in E135 nodules we studied the nitrogenase activities in isolated bacteroids and in cell-free extracts of bacteroids, and measured the two components of nitrogenase protein in bacteroids. Bacteroids prepared anaerobically from E135 nodules showed no acetylene reduction activity in the presence and absence of myoglobin. Furthermore, no acetylene reduction activity by cell-free extracts of E135 bacteroids was detected in the presence of ATP-generating system and dithionite. However, immunoblotting analyses revealed the presence of nitrogenase components I and II in E135 nodule bacteroids. These results suggest that a host plant gene is involved in the expression of nitrogenase activity in symbiotic bacteria.
- 日本植物生理学会の論文
著者
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HAYASHI Kenji
Department of Energy Science and Engineering, Kyoto University
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KOUCHI Hiroshi
Department of Plant Physiology, National Institute of Agrobiological Sciences
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Kouchi Hiroshi
Nati. Inst. Agrobiol. Res.
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Kouchi Hiroshi
Natio. Insti. Agrobiol. Res. Tsukuba Naist
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Kouchi Hiroshi
National Institute Of Agrobiological Resources Tsukuba Science City
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Hayashi Keiko
Hokuriku National Agri. Exp. Station
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SUGANUMA Norio
Department of Agronomy, School of Agriculture, Nagoya University
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Suganuma Norio
Dept. Life Sci. Aichi Univ.
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Suganuma Norio
Department Of Agronomy School Of Agriculture Nagoya University
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Tamaoki Masanori
Dept. Life Sci. Aichi Univ.
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Tamaoki Masanori
Department Of Life Science Aichi University Of Education
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Kouchi Hiroshi
Department Of Applied Physiology National Institute Of Agrobiological Resources
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HAYASHI Takamitsu
Department of Pure and Applied Chemistry, Faculty of Science and Technology, Tokyo University of Sci
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Hayashi Kenji
Hokuriku National Agri. Exp. Station
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Sonoda Nobutaka
Department of Life Science, Aichi University of Education
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Nakane Chizuru
Department of Life Science, Aichi University of Education
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Nakane Chizuru
Department Of Life Science Aichi University Of Education
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Sonoda Nobutaka
Department Of Life Science Aichi University Of Education
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Hayashi Takamitsu
Department Of Life Science Aichi University Of Education
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Kouchi Hiroshi
Department Of Agricultural Chemistry University Of Tokyo
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Hayashi Kenji
Department Of Dermatology Tokyo Women's Medical University
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