Variation in the Content and Composition of Sterols in Alfalfa Seedlings Treated with Compactin (ML-236B) and Mevalonic Acid

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Compactin (ML-236B), a specific inhibitor of 3-hydroxy-3-methylglutaryl COA reductase, inhibited the elongation of roots and hypocotyls of Medicago saliva seedlings when it was applied to the roots. Addition ofmevalonic acid, the direct product ofthe enzyme, together with compactin relieved the growth inhibition of roots. The contents and compositions of sterols were studied in three parts of M. sativa seedlings-roots, hypocotyls and cotyledons. Compactin (20 pM) decreased the sterol contents of roots and hypocotyls by about a half but did not affect that of cotyledons. On the other hand, mevalonic acid (2 mM) increased the sterol content of roots more than threefold the nontreated control level but not the contents of hypocotyls and cotyledons. Mevalonic acid added in combination with compactin had a similar effect on the sterol content of roots as when it was added alone. The major sterol in all three parts was stigmasterol whether or not compactin or mevalonic acid was present. However, the variation of the sterol composition in the roots was distinct; mevalonic acid-treated roots markedly accumulated A7-sitosterol, 24-methylenecycloartanol and squalene.
日本植物生理学会の論文