Accumulation of Rare Phytosterols in Plant Cells on Treatment with Metabolic Inhibitors and Mevalonic Acid
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概要
- 論文の詳細を見る
Root application of the metabolic inhibitors D-ethionine (100μM) and L-ethionine (30μM), which were expected to inhibit alkylation of the sterol side chains, merely reduced the sterol content in the roots of Medicago sativa seedlings. The major sterol was stigmasterol. However, when (3RS)-mevalonic acid (2mM) was applied together with ethionine, cycloartenol (about 50% of the total sterols) accumulated in the roots. The hypocotyls of the ethionine-treated seedlings accumulated cholesterol (34% of the total sterols), and mevalonic acid showed no additional effect in this case. In a suspension culture of Nicotiana tabacum, the most abundant sterol was campesterol. When cells were treated with buthiobate (100μM), a potent inhibitor of lanosterol 14α-demethylation in yeasts and fungi, obtusifoliol accumulated in the treated cells (50% of the total sterols). 14α-Methylfecosterol also accumulated in the treated cells. The addition of mevalonic acid (1mM) together with buthiobate increased the obtusifoliol content (63% of the total sterols).
- 日本植物生理学会の論文
著者
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Kouchi Hiroshi
National Institute Of Agrobiological Sciences
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Kouchi Hiroshi
Nati. Inst. Agrobiol. Res.
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Kouchi Hiroshi
Natio. Insti. Agrobiol. Res. Tsukuba Naist
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Kouchi Hiroshi
National Institute Of Agrobiological Resources Tsukuba Science City
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Takano Hiroyuki
Agriculture Forestry And Fisheries Technical Information Society
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SHIRATA Kazuto
National Institute of Agrobiological Sciences
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Hata Shingo
Laboratory Of Plant Physiology Division Of Applied Biosciences Graduate School Of Agriculture Kyoto
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Hata Shingo
Laboratory Of Plant Pathology Division Of Environmental Science Of Bioresource Production Graduate S
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Hata Shingo
National Institute of Agrobiological Resources, Tsukuba Science City
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Takagishi Hidejiro
National Institute of Agrobiological Resources, Tsukuba Science City
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Takagishi H
National Inst. Agrobiological Resources Ibaraki Jpn
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Takagishi Hidejiro
National Institute Of Agrobiological Resources Tsukuba Science City
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Hata Shingo
National Institute Of Agrobiological Resources Tsukuba Science City
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Takagishi Hidejiro
National Institute of Agrobiological Resources
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