Long-term conservation of Diospyros germplasm using dormant buds by a prefreezing method
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概要
- 論文の詳細を見る
Cryopreservation of Diospyros at-150°C followed by a prefreezing treatment using dormant winter buds was investigated. Twig sections bearing one bud each were first partly dehydrated at room temperature for 3 h. Then they were subjected to a successive prefreezing at-5, -10, -15, -20 and-30°C at 24 h intervals, and finally preserved at-150°C in a deep freezer. Thawing in the air at 25°C gave the highest rate (about 70%) of in vitro shoot formation from cryopreserved buds. Shoot regeneration by bud grafting was not successful. Dormant buds appear to be a suitable material for long-term storage of some Diospyros species.
- 日本植物細胞分子生物学会の論文
- 2004-09-01
著者
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Matsumoto Shingo
Shimane Univ. Shimane Jpn
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Matsumoto Shingo
Shimane Univ. Fac. Of Life And Environmental Science
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Matsumoto Toshikazu
Shimane Agricultural Experimental Station
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NIINO Takao
National Institute of Agrobiological Sciences
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SHIRATA Kazuto
National Institute of Agrobiological Sciences
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KURAHASHI Takao
Shimane Agricultural Experiment Station
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MAKI Shinya
Chuden Kankyo Technos
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ITAMURA Hiroyuki
Shimane Univ., Fac. Of Life and Environmental Science
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Matsumoto Shingo
Shimane Agricultural Experiment Station
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Kurahashi Takao
Shimane Agricultural Technology Center
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Itamura Hiroyuki
Faculty Of Life And Environmental Science Shimane University
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Matsumoto Toshikazu
Shimane Agricultural Technology Center
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Matsumoto Toshikazu
Shimane Agricultural Experiment Station
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NIINO Takao
National Agricultural Research Center for Tohoku Region
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