Molecular Cloning and Characterization of a cDNA Clone That Encodes a Cdc2 Homolog from Nicotiana tabacum
スポンサーリンク
概要
- 論文の詳細を見る
We have isolated a cDNA clone (cdc2Nt1) that encodes a homolog of p34^<cdc2/CDC28> kinase from tobacco (Nicotiana tabacum). The cdc2Nt1 protein showed extensive similarity to other homologs of Cdc2 from plants. Complementation studies showed that the cdc2Nt1 gene was able to overcome cell cycle arrest at both the G1/S and the G2/M transitions of cdc28^<ts> mutants of budding yeast, demonstrating that the cdc2Nt1 protein was able to replace the Cdc28 kinase at both the G1/S and the G2/M transitions. Analysis of gene expression demonstrated that the cdc2Nt1 gene was transcribed constitutively throughout the cell cycle but that it was preferentially expressed in actively dividing tobacco BY-2 cells.
- 日本植物生理学会の論文
著者
-
SHINMYO ATSUHIKO
Graduate School of Biological Sciences, Nara Institute of Science and Technology
-
Kouchi Hiroshi
National Institute Of Agrobiological Sciences
-
Kouchi Hiroshi
Nati. Inst. Agrobiol. Res.
-
Kouchi Hiroshi
Natio. Insti. Agrobiol. Res. Tsukuba Naist
-
Kouchi Hiroshi
National Institute Of Agrobiological Resources Tsukuba Science City
-
Shinmyo Atsuhiko
Graduate School Of Biological Science Nara Institute Of Science And Technology
-
Shinmyo Atsuhiko
Nara Insti. Sci. Technol. Nara
-
関根 政実
奈良先端大・バイオ
-
Setiady Yulius
Department Of Biotechnology Faculty Of Engineering Osaka University
-
Sekine Masami
Graduate school of Biological sciences, Nara Institute of science and Technology
-
Hariguchi Norimitsu
Department of Biotechnology, Faculty of Engineering, Osaka University
-
関根 政実
Department Of Bioproduction Science Faculty Of Bioresources And Environmental Sciences Ishikawa Pref
-
Sekine M
Waseda Univ. Tokyo Jpn
-
Hariguchi Norimitsu
Department Of Biotechnology Faculty Of Engineering Osaka University
関連論文
- The 5'-end expressed sequence tags of Lotus japonicus
- Improving salt tolerance in plant cells
- Introduction of the Archaebacterial Geranylgeranyl Pyrophosphate Synthase Gene into Chlamydomonas reinhardtii Chloroplast
- Cadmium-tolerance of transgenic Ipomoea aquatica expressing serine acetyltransferase and cysteine synthase
- Simultaneous expression of serine acetyltransferase and cysteine synthase results in enhanced sulfate uptake and increased biomass in Ipomaea aquatica
- Efficient Plant Regeneration of Ipomoea aquatica by Direct Shoot Formation from Cotyledon Segments
- URICASE GENE EXPRESSION IN A NON-ALLANTOIN TYPE LEGUME, MEDICAGO SATIVA
- Effect of the sequence context of the AUG initiation codon on the rate of translation in dicotyledonous and monocotyledonous plant cells(PLANT BIOTECHNOLOGY)
- The 5'-Untranslated Region of the Oryza sativa Alcohol Dehydrogenase Gene Functions as a Translational Enhancer in Monocotyledonous Plant Cells(PLANT BIOTECHNOLOGY)
- Preferential Translation Mediated by Hsp81-3 5'-UTR during Heat Shock Involves Ribosome Entry at the 5'-end Rather than an Internal Site in Arabidopsis Suspension Cells(PLANT BIOTECHNOLOGY)
- The Lotus Symbiont, Mesorhizobium loti: Molecular Genetic Techniques and Application
- Quantitative Trait Locus Analysis of Flowering Time in Soybean Using a RFLP Linkage Map
- cDNA Cloning of Squalene Synthase Genes from Mono- and Dicotyledonous Plants, and Expression of the Gene in Rice
- ON THE STRUCTURE AND EXPRESSION OF PLANT SQUALENE SYNTHASE
- The Arabidopsis Genome Project; Current Status of Sequencing and Re-annotation
- LjnsRING, a Novel RING Finger Protein, is Required for Symbiotic Interactions Between Mesorhizobium loti and Lotus japonicus
- Characterization of Uricase Promoter : GUS Gene Expression Using transgenic Lotus japonicus
- Characterization of Uricase Proteins Expressed Using pET32 and Soybean Nodule cDNA Clones
- Characterization of Transgenic Lotus japonicus Containing Sense and Anti-sense Uricase Genes
- Symbiotic Nitrogen Fixation at the Late Stage of Nodule Formation in Lotus japonicus and Other Legume Plants
- Expression of Two Uricase (Nodulin-35) Genes in a Non-Ureide Type Legume, Medicago sativa
- PROMOTER ANALYSIS OF URICASE II (NODULIN 35) GENES TO IDENTIFY THE ELEMENTS FOR NODULE UNINFECTED CELL-SPECIFIC EXPRESSION
- Comparison of uricase II (nodulin 35) gene promoters from soybean, alfalfa and non-legume, Arabidopsis.
- ISOLATION AND CHARACTERIZATION OF AN URICASE II (NODULIN 35) GENE FROM ARABIDOPSIS.
- URICASE (NOD-35) GENE EXPRESSION ANALYSIS IN UREIDE AND AMIDE TYPE LEGUME PLANTS
- ANALYSES OF THE PROMOTER REGION OF THE SOYBEAN URICASE GENES USING TRANSGENIC HAIRY ROOT SYSTEM.
- Mapping and Characterization of DNase I Hypersensitive Sites in Arabidopsis Chromatin
- Tobacco RETINOBLASTOMA-RELATED protein is phosphorylated by different types of cyclin-dependent kinases during the cell cycle
- Cloning and Expression Analysis of a MAPKKK Gene and a Novel Nodulin Gene of Lotus japonicus
- Molecular Cloning and Characterization of a cDNA Clone That Encodes a Cdc2 Homolog from Nicotiana tabacum
- Expression of OsHAK genes encoding potassium ion transporters in rice
- Functions of HKT transporters in sodium transport in roots and in protecting leaves from salinity stress
- High-Efficiency Secretory Production of Peroxidase Cla Using Vesicular Transport Engineering in Transgenic Tobacco(PLANT BIOTECHNOLOGY)
- TWO TYPES OF HKT HOMOLOGUES WITH DIFFERENT PROPERTIES OF Na^+ AND K^+ TRANSPORTATION IN Oryza sativa
- TRANSCRIPTIONAL REGULATION OF RICE HKT1 GENE BY K^+, Na^+
- Resistance to insects in transgenic Solanum plants expressing a peroxidase gene from horseradish
- Phylogenetic profiling of Arabidopsis genes by monitoring spatial gene expression using cDNA microarrays
- Expression of Randomly Integrated Single Complete Copy Transgenes Does not Vary in Arabidopsis thaliana
- Systematic Transient Assays of Promoter Activities for Leaf-Specific Genes Identified by Gene-Expression Profiling with cDNA Microarrays in Arabidopsis thaliana(PLANT BIOTECHNOLOGY)
- Enhanced Resistance to Blast Fungus and Bacterial Blight in Transgenic Rice Constitutively Expressing OsSBP, a Rice Homologue of Mammalian Selenium-binding Proteins
- Efficient Construction of cDNA Microarrays Utilizing Normalized cDNA Libraries of Arabidopsis thaliana
- Wound-responsive cis-Element in the 5'-Upstream Region of Cucumber Ascorbate Oxidase Gene(Biochemistry & Molecular Biology)
- 5'Untranslated Region of the HSP 18.2 Gene Contributes to Efficient Translation in Plant Cells(PLANT BIOTECHNOLOGY)
- Isolation of Growth-Phase-Specific Promoters from Cultured Tobacco Cells
- Expression of a Foreign Gene in Chlamydomonas reinhardtii Chloroplast
- Expression of a Gene for Uricase II (Nodulin-35) in Cotyledons of Soybean Plants
- Transgene Expression Systems in Plant, a Natural Bioreactor
- A Supernodulating Mutant Isolated from Soybean Cultivar Enrei
- Transcriptional Response of Soybean Suspension-cultured Cells Induced by Nod Factors Obtained from Bradyrhizobium japonicum USDA110
- Isolation and Characterization of a cDNA That Encodes a Novel Proteinase Inhibitor I from a Tobacco Genetic Tumor
- Accumulation of Rare Phytosterols in Plant Cells on Treatment with Metabolic Inhibitors and Mevalonic Acid
- Variation in the Content and Composition of Sterols in Alfalfa Seedlings Treated with Compactin (ML-236B) and Mevalonic Acid
- PROGRESS IN Arabidopsis thaliana GENOME SEQUENCING PROJECT
- Improvement on Production of (R)-4-Chloro-3-Hydroxybutyrate and (S)-3-Hydroxy-γ-Butyrolactone with Recombinant Escherichia coli Cells
- cDNA cloning and expression of cyclin D gene in Nicotiana tabacum
- Streptomyces-Derived Induction System for Gene Expression in Cultured Plant Cells(PLANT BIOTECHNOLOGY)
- How Many Peas in a Pod? Legume Genes Responsible for Mutualistic Symbioses Underground
- Cloning, Functional Expression, and Mutational Analysis of a cDNA for Lotus japonicus Mitochondrial Phosphate Transporter
- Characterization of a Saccharomyces cerevisiae Gene That Encodes a Mitochondrial Phosphate Transporter-Like Protein^1
- cDNA CLONING OF A MITOCHONDRIAL PHOSPHATE TRANSPORTER GENE FROM Lotus japonicus
- cDNA CLONING AND CHARACTARIZATION OF Lotus japonicus GENES FOR SQUALENE SYNTHASE
- PROMOTER ANALYSIS OF THE TWO SOYBEAN PEPC GENES : REGULATORY ELEMENTS AND NUCLEAR FACTORS REQUIRED FOR NODULE-ENHANCED EXPRESSION
- cDNA CLONING AND CHARACTERIZATION OF PHOSPHOENOLPYRUVATE CARBOXYLASE AND ITS PROTEIN KINASE OF Lotus japonicus ROOT NODULES
- EXPRESSION OF GENES ENCODING NODULINS CONTAINING CONSERVED CYSTEINE CLUSTERS IN PEA NODULES
- Sites of Expression of DnaJ Homologs and Hsp 70 in Male and Female Flowers of the Japanese Willow Salix gilgiana Seemen
- Male Flower-Specific Expression of Genes for Polygalacturonase, Pectin Methylesterase and β-1, 3-Glucanase in a Dioecious Willow(Salix gilgiana Seemen)
- Promoter analysis of a soybean nodule-enhanced PEPC gene
- Genome-Wide Analyses of Early Translational Responses to Elevated Temperature and High Salinity in Arabidopsis thaliana
- The HSP Terminator of Arabidopsis thaliana Increases Gene Expression in Plant Cells
- Ribulose 1,5-Bisphosphate Carboxylase/Oxygenase Large Subunit Translation is Regulated in a Small Subunit-Independent Manner in the Expanded Leaves of Tobacco
- Efficient Translation Destabilizes Transcripts in Chloroplasts of Chlamydomonas reinhardtii (PLANT BIOTECHNOLOGY)
- Production of(S)-4-Chloro-3-Hydroxybutyrate by Microbial Resolution Using Hydrolase from Rhizobium sp. DS-S-51(BIOCHEMICAL ENGINEERING)
- An E2F-Regulated Reporter Construct is Transcriptionally Activated Following the Transient Expression of Cyclin D in Plants
- The 5'-Untranslated Region of the Tobacco Alcohol Dehydrogenase Gene Functions as an Effective Translational Enhancer in Plant(PLANT BIOTECHNOLOGY)
- Function of GRAS Proteins in Root Nodule Symbiosis is Retained in Homologs of a Non-Legume, Rice
- Determination of the in vivo distribution of nuclear matrix attachment regions using a polymerase chain reaction-based assay in Arabidopsis thaliana(GENETICS, MOLECULAR BIOLOGY AND GENE ENGINEERING)
- HETEROGENEITY OF PEA ISOLEGHEMOGLOBINS
- Bacteroids Isolated from Ineffective Nodules of Pisum sativum Mutant E135 (sym13) Lack Nitrogenase Activity but Contain the Two Protein Components of Nitrogenase
- Rice sodium-insensitive potassium transporter, OsHAK5, confers increased salt tolerance in tobacco BY2 cells(PLANT BIOTECHNOLOGY)
- Expression of two uricase genes in various tissues of soybean plants
- A novel pathosystem to study the interactions between Lotus japonicus and Fusarium solani
- Molecular cloning and expression of a cDNA clone for Rb from Nicotiana tabacum
- CHARACTERIZATION OF NODULES FORMED BY Rhizobium etli ON Lotus japonicus ROOTS
- RNAi Knock-Down of ENOD40s Leads to Significant Suppression of Nodule Formation in Lotus japonicus
- Improving salt tolerance in plant cells
- THE Arabidopsis GENOME PROJECT III. SEQUENCE ANALYSIS OF CHROMOSOME III AND V
- THE Arabidopsis GENOME PROJECT IV : A LARGE SCALE cDNA SEQUENCING
- Arabidopsis genome sequencing project II. Construction of Arabidopsis thaliana genome data opening system
- Arabidopsis GENOME SEQUENCING PROJECT I. PHYSICAL MAP CONSTRUCTION AND DNA SEQUENCING STRATEGY
- OCCURRENCE OF UREIDE ACCUMULATION IN SOYBEAN PLANTS : Effect of Nitrogen Fertilization and N_2 Fixation
- EXPRESSION OF GENES FOR PECTINASES IN REPRODUCTIVE ORGANS OF A WILLOW (Salix gilgiana Seemen)
- 水生植物パックブン(Ipomoea aquatica)節組織からの植物体の再生
- Effects of Gamma-irradiation on Lipid Metabolic Changes of Potato Tubers in Response to Mechanical Injury
- MOLECULAR CLONING OF cDNAs FOR PLANT MITOCHONDRIAL PHOSPHATE TRANSPORTER
- EXPRESSION OF PHOSPHOENOLPYRUVATE CARBOXYLASE IN NITROGEN-FIXING NODULES OF SOYBEAN ROOTS
- Characterization of the Rice Blast Fungal Elicitor-Responsive Gene OsSBP Encoding a Homolog to the Mammalian Selenium-binding Proteins
- Plant biotechnology : a key technology in the 21st century
- An E2F-Regulated Reporter Construct is Transcriptionally Activated Following the Transient Expression of Cyclin D in Plants.
- The Integral Membrane Protein SEN1 is Required for Symbiotic Nitrogen Fixation in Lotus japonicus Nodules
- Rj(rj) genes involved in nitrogen-fixing root nodule formation in soybean