Resistance to insects in transgenic Solanum plants expressing a peroxidase gene from horseradish
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概要
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To engineer insect-resistant Solanum plants, a peroxidase C2 gene (prxC2) from horseradish was introduced into Solanum integrifolium Poir. cv. Hiranasu. We produced 77 transgenic Hiranasu plants. Peroxidase expression was confirmed at the transcriptional and translational levels by northern blot analysis and by measuring peroxidase activity, respectively. Feeding test results show that transformant no. 180 is more resistant to corn earworm (Heliothis armigera) and common cutworm (Spodoptera litura) than the wild-type plants. We also found a correlation between insect resistance and lignin content in the transgenic plants. In particular, the lignin content of transformant no. 180 was 30% higher than that of wild-type plants. These results further confirm that peroxidase is functionally expressed in transgenic plants and suggest that the increased lignin content is a basis for the insect resistance in transgenic Hiranasu plants.
- 日本植物細胞分子生物学会の論文
- 2006-03-01
著者
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新名 惇彦
阪大工
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TAKANO Toshiyuki
Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University
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NAKATSUBO Fumiaki
Division of Forest and Biomaterials Science, Graduate School of Agriculture, Kyoto University
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Shinmyo A
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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新名 惇彦
奈良先端科学技術大学院大学バイオサイエンス研究科
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SHINMYO ATSUHIKO
Graduate School of Biological Sciences, Nara Institute of Science and Technology
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Shinmyo Atsuhiko
Graduate School Of Biological Science Nara Institute Of Science And Technology
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Shinmyo Atsuhiko
Nara Insti. Sci. Technol. Nara
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Shinmyo Atsuhiko
Department Of Biotechnotogy Faculty Of Engineering Osaka University:department Of Bioscience Nara In
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Yoshida Kazuya
Graduate School Of Biological Sciences Nara Institute Of Science And Technology
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Takano Toshiyuki
Division Of Forest And Biomaterials Science Graduate School Of Agriculture Kyoto University
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Nakatsubo Fumiaki
Division Of Forest And Biomaterials Science Graduate School Of Agriculture Kyoto University
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TSUDUKI Masao
Nara Prefectural Agricultural Experiment Station
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ASAO Hiroshi
Nara Prefectural Agricultural Experiment Station
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新名 惇彦
奈良先端大
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