Comparison of the Protein Composition and Enzymatic Activities during Development between Effective and Plant-Determined Ineffective Nodules in Pea

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The protein composition and enzymatic activities during development of ineffective nodules, produced by mutant E135 (sym 13) of pea (Pisum sativum L.), were compared with those of the nitrogen-fixing nodules of the normal parent, the Sparkle cultivar. The protein composition of 3-week-old E135 nodules, as determined by SDS-polyacrylamide gel electrophoresis, was quite similar to that of Sparkle nodules. After 4 weeks, however, the intensities of bands of 15-, 38-, and 87-kDa polypeptides were lower in the case of E135 nodules. Western blot analysis using a "nodule-specific" antiserum revealed that most nodulins could be detected in 3-week-old E135 nodules, but a 35.5-kDa nodulin disappeared after 5 weeks and several novel peptides ranging in molecular weight from 26 to 31 kDa appeared after 6 weeks in E135 nodules. The activities of glutamine synthetase, glutamate synthase, alanine-pyruvate aminotransferase, sucrose synthase, and phosphoenolpyruvate carboxylase increased during development of Sparkle nodules, but such increases were not found in E135 nodules after 5 weeks. These results show that the nodules of E135 begin to develop normally but differ from those of Sparkle within 4 weeks, indicating that, during early stages of nodule development, the protein composition and activities of enzymes involved in carbon and nitrogen metabolism are not regulated by the presence or absence of nitrogenase activity.
日本植物生理学会の論文