Detection of Polypeptides Involved in Early Stages of Nodulation in Soybean Roots

概要

論文の詳細を見る
Soluble proteins extracted from the roots of nodulating soybean [Glycine max (L.) Merr. cv. T202] and from roots of the non-nodulating isoline rj_1 of cv. T202 (cv. T201), which had been inoculated with Bradyrhizobium japonicum, were analyzed by two-dimensional polyacrylamide gel electrophoresis and silver staining, in an attempt to identify polypeptides involved in early stages of nodulation. Almost identical patterns of polypeptides were generated by extracts of 3-day-old roots of uninoculated T201 and T202 and of inoculated T201 and T202, but a unique spot, corresponding to a polypeptide of 38 kDa was detected in the case of inoculated roots of T202. Western blotting analysis using "inoculated-T202-root specific" antiserum, prepared by titration of antiserum against proteins from inoculated T202 roots with proteins from inoculated T201 roots, revealed spots corresponding to polypeptides of 26, 27, and 33 kDa that were detectable only in the extracts of roots of inoculated T202. However, no unique polypeptide spots were detected in the case of roots of inoculated T201 and T202, as compared with those from uninoculated T201 and T202 roots by Western blotting analysis using "inoculated-T201-root specific" antiserum prepared by titration of antiserum against proteins from inoculated T201 roots with proteins from uninoculated T201 roots.
日本植物生理学会の論文