Expression and Complementation Analyses of a Chloroplast-Localized Homolog of Bacterial RecA in the Moss Physcomitrella patens

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概要

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• RecA protein is widespread in bacteria, and it plays a crucial role in homologous recombination. We have identified two bacterial-type recA gene homologs (PprecA1, PprecA2) in the cDNA library of the moss Physcomitrella patens. N-terminal fusion of the putative organellar targeting sequence of PpRecA2 to the green fluorescent protein (GFP) caused a targeting of PpRecA2 to the chloroplasts. Mutational analysis showed that the first AUG codon acts as initiation codon. Fusion of the full-length PpRecA2 to GFP caused the formation of foci that were colocalized with chloroplast nucleoids. The amounts of PprecA2 mRNA and protein in the cells were increased by treatment with DNA damaging agents. PprecA2 partially complemented the recA mutation in Escherichia coli. These results suggest the involvement of PpRecA2 in the repair of chloroplast DNA.

• 社団法人 日本農芸化学会の論文
• 2008-05-23

著者

• Hasebe Mitsuyasu

  National Institute For Basic Biology

• Fujita Tomomichi

  Department Of Biological Sciences Faculty Of Science Hokkaido University

• Sekine Yasuhiko

  Department Of Life Science College Of Science Rikkyo (st. Paul's) University

• Inouye Takayuki

  Department Of Life Science College Of Science Rikkyo (st. Paul's) University

• Odahara Masaki

  Department Of Life Science College Of Science Rikkyo (st. Paul's) University

• FUJITA Tomomichi

  National Institute for Basic Biology

• Odahara Masaki

  Department Of Life Science College Of Science Rikkyou University

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