Nucleotide sequence variation was unexpectedly low in an endangered species, Aldrovanda vesiculosa L. (Droseraceae)
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概要
- 論文の詳細を見る
Using fluorescence in situ hybridization (FISH) and polymerase chain reaction direct sequence methods, 45S ribosomal DNA (rDNA) loci on somatic chromosomes were detected and partial sequence of 45S rDNA was determined in Aldrovanda vesiculosa, respectively. Analysis of FISH showed tree to five 45S rDNA signals in interphase nuclei, and four signals at metephase stage. All FISH signals at metaphase stage were located at the terminal region of four chromosomes. Among six populations, completely homologue sequence result was supplied from Clustal W analysis. After determining sequences of 5.8S and internal transcribed spacer (ITS) in 45S rDNA of six populations, a comparative study with these sequences was carried out to infer genetic diversity in A. vesiculosa.
- 国際染色体植物学会の論文
著者
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Hasebe Mitsuyasu
National Institute For Basic Biology
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Hoshi Yoshikazu
Department Of Plant Science Kyushu Tokai University
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Hoshi Yoshikazu
Department of Plant Science, School of Agriculture, Kyushu Tokai University
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Shirakawa Junichi
Department of Plant Science, School of Agriculture, Kyushu Tokai University
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