Cloning and Characterization of a NADP^+ -Malic Enzyme Gene from Bradyrhizobium japonicum USDA110
スポンサーリンク
概要
- 論文の詳細を見る
Malic enzymes have been considered to play a key role in energy metabolism for nitrogenase reaction in bacteroids. To elucidate the physiological role of the malic enzymes in Bradyrhizobium japonicum bacteroids, a putative malic enzyme gene Bjtme1 was cloned by polymerase chain reaction (PCR) using degenerated primers from conserved regions of the protein sequences of bacterial malic enzymes and draft sequence data of the Bradyrhizobium japonicum USDA110 genome sequence project. To confirm the characteristics of the Bjtme1 gene, the protein encoded by this gene was over-expressed using a pET32a(+) system and it exhibited a NADP^+ -malic enzyme (EC 1.1.1.40) activity, indicating that Bjtme1 was the gene of the NADP^+ -malic enzyme. This is the first report on the cloning and characterization of the NADP^+ -malic enzyme gene from B. japonicum, and the gene structure was compared with that of NADP^+ -malic enzyme genes of other rhizobia.
- 社団法人日本土壌肥料学会の論文
著者
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TABATA Satoshi
Kazusa DNA Research Institute
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Nomura Mika
Faculty of Agriculture, Kagawa University
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Tajima Shigeyuki
Faculty of Agriculture, Kagawa University
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NOMURA Mika
Department of Life Science, Faculty of Agriculture, Kagawa University
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KURIYAMA Tomoko
RIKEN Plant Science Center
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Tabata Satoshi
Kazusa Dna Res. Inst. Chiba Jpn
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Kaneko Takakazu
Kazusa Dna Research Institute
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Kaneko Takakazu
Department Of Life Science Faculty Of Agriculture Kagawa University
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Kinoue Tomoyo
Kagoshima Univ. Kagoshima Jpn
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Tabata S
Kazusa Dna Res. Inst. Chiba Jpn
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TAJIMA Shigeyuki
Kagawa Univ.
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Nomura Mika
Faculty Of Agriculture Kagawa University
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Nomura Mika
Department Of Hypertension And Cardiorenal Medicine Dokkyo Medical University School Of Medicine
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Nomura Mika
Deppartment Of Life Sciences Faculty Of Agriculture Kagawa University
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Nomura M
Kansai Electric Power Co. Inc. Amagasaki Jpn
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Nomura Mika
Fac. Agr. Kagawa University
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Nomura Mika
Department Of Life Sciences Faculty Of Agriculture Kagawa University
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Tabata Satoshi
Kazusa Dna Res.
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Tajima Shigeyuki
Kagawa Univ. Kagawa Jpn
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Tajima Shigeysuki
Department Of Life Science Faculty Of Agriculture Kagawa University
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Kato Tomohiko
Mitsui Plant Biotech. Res. Inst.
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SINSUWONGWAT SUPHAWAT
Department of Biotechnology, Faculty of Agro-Industry, Chiangmai University
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Tanaka K
Kazusa Dna Research Institute
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Tajima Shigeyuki
Faculty Of Agriculture Kagawa University
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Kodera Amane
Department of Life Science, Faculty of Agriculture, Kagawa University
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Tajima S
Faculty Of Agriculture Kagawa University
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Tajima S
Department Of Life Science Kagawa University
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Tajima Shigeyuki
Deppartment Of Life Sciences Faculty Of Agriculture Kagawa University
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Kodera Amane
Department Of Life Science Faculty Of Agriculture Kagawa University
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Nomura M
Faculty Of Agriculture Kagawa University
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Tajima Shigeyuki
Fac. Agr. Kagawa University
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Tajima Shigeyuki
Dept. Of Bioresource Science Faculty Of Agriculture Kagawa University
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Sinsuwongwat Suphawat
香大農
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Tajima Shigeyuki
Department Of Life Sciences Faculty Of Agriculture Kagawa University
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Takegawa Kaoru
Department Of Life Science Faculty Of Agriculture Kagawa University
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Sinsuwongwat Suphawat
Department Of Biotechnology Faculty Of Agro-industry Chiangmai University
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Nomura Mika
Faculty Of Agriculture Kagawa University Kagawa
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Kiba T
Laboratory Of Molecular Microbiology School Of Agriculture Nagoya University
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Takeda S
Kazusa Dna Research Institute
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Kaneko Takakazu
Kazusa Dna Res. Inst.
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Tabata S
Kazusa Dna Res. Inst. Chiba
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Tabata Satoshi
Kazusa Dna Institute
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