Characterization of Local Rhizobia in Thailand and Distribution of Malic Enzymes

スポンサーリンク

概要

• 論文の詳細を見る

• Twenty-six isolates were obtained from nodules of various legume plants (Glycine max, Vigna sinensis, Arachis hypogaea, Desmanthus virgatus, Acacia mangium, Centrosema pascuorum, Pterocarpus indicus, Xylia xylocarpa, and Sesbania rostrata) in Thailand. After confirming their nodulation and nitrogen-fixing abilities, they were identified by 16S rRNA gene analysis as Bradyrhizobium japonicum, Bradyrhizobium elkanii, Rhizobium leguminosarum, Rhizobium gallicum, and Rhizobium galegae. Using these local isolates, the distribution of the activities of both NAD^+ -dependent (DME: EC 1.1.1.39) and NADP^+ -dependent (TME: EC 1.1.1.40) malic enzymes was surveyed. The malic enzyme activities were present in all the isolated rhizobia and in other 17 local Bradyrhizobium strains in Thailand. In almost all the rhizobia, the DME activity predominated whereas the TME activity predominated only in the Rhizobium gallicum strains that were major symbionts of Sesbania rostrata. Southern hybridization analysis was performed to survey the distribution of the malic enzyme genes among the local rhizobia, which are similar to those of B. japonicum. DNA probes (ME1 for DME and ME2 for TME) were prepared by polymerase chain reaction (PCR) using degenerated primers from conserved regions of the protein sequences of bacterial malic enzymes. Southern blot analysis with ME1 as a probe showed a single band in about half of the isolates, especially in B. japonicum and R. leguminosarum strains, suggesting the wide distribution of such DME genes among local rhizobia. In contrast, Southern blot analysis with ME2 as a probe detected a single band only in five B. japonicum strains, suggesting that the TME genes, which are similar to those of B. japonicum, would be unique in a group of B. japonicum.

• 社団法人日本土壌肥料学会の論文

著者

• Tajima Shigeyuki

  Department of Bioresource Science, Faculty of Agriculture, Kagawa University

• Nomura Mika

  Faculty of Agriculture, Kagawa University

• Tajima Shigeyuki

  Faculty of Agriculture, Kagawa University

• NOMURA Mika

  Department of Life Science, Faculty of Agriculture, Kagawa University

• Shutsrirung Arawan

  Faculty Of Agriculture Chiangmai University

• TAJIMA Shigeyuki

  Kagawa Univ.

• Nomura Mika

  Faculty Of Agriculture Kagawa University

• Nomura Mika

  Department Of Hypertension And Cardiorenal Medicine Dokkyo Medical University School Of Medicine

• Nomura Mika

  Deppartment Of Life Sciences Faculty Of Agriculture Kagawa University

• Nomura M

  Kansai Electric Power Co. Inc. Amagasaki Jpn

• Nomura Mika

  Fac. Agr. Kagawa University

• Nomura Mika

  Department Of Life Sciences Faculty Of Agriculture Kagawa University

• Tajima Shigeyuki

  Kagawa Univ. Kagawa Jpn

• Tajima Shigeysuki

  Department Of Life Science Faculty Of Agriculture Kagawa University

• SINSUWONGWAT SUPHAWAT

  Department of Biotechnology, Faculty of Agro-Industry, Chiangmai University

• Tajima Shigeyuki

  Faculty Of Agriculture Kagawa University

• Nuntagij Achara

  Biologicai Nitrogen Fixation Resource Center, Soil Science Division, Department of Agriculture

• Tajima S

  Faculty Of Agriculture Kagawa University

• Tajima S

  Department Of Life Science Kagawa University

• Tajima Shigeyuki

  Deppartment Of Life Sciences Faculty Of Agriculture Kagawa University

• Tajima Shigeyuki

  Department Of Agricultural Chemistry Faculty Of Agriculture Nagoya University

• Nuntagij Achara

  Biologicai Nitrogen Fixation Resource Center Soil Science Division Department Of Agriculture

• Nomura M

  Faculty Of Agriculture Kagawa University

• Tajima Shigeyuki

  Fac. Agr. Kagawa University

• Tajima Shigeyuki

  Dept. Of Bioresource Science Faculty Of Agriculture Kagawa University

• Sinsuwongwat Suphawat

  香大農

• Tajima Shigeyuki

  Department Of Life Sciences Faculty Of Agriculture Kagawa University

• Sinsuwongwat Suphawat

  Department Of Biotechnology Faculty Of Agro-industry Chiangmai University

• Nomura Mika

  Faculty Of Agriculture Kagawa University Kagawa

• Tajima Shigeyuki

  Dept. Bioresource Sience Kagawa Univ.

関連論文

• Purification and Characterization of D-Tagatose 3-Epimerase from Pseudomonas sp. ST-24
• The 5'-end expressed sequence tags of Lotus japonicus
• 8-20 Screening of Mesorhizobium loti mutants for symbiotic N_2 fixation in Lotus japonicus
• Identification of a Sed5-like SNARE Gene LjSYP32-1 that Contributes to Nodule Tissue Formation of Lotus japonicus
• Differential Expression Pattern of C_4 Bundle Sheath Expression Genes in Rice, a C_3 Plant
• The Promoter for C_4-type Mitochondrial Aspartate Aminotransferase Does not Direct Bundle Sheath-specific Expression in Transgenic Rice Plants
• High Level Expression of C_4-Specific NADP-Malic Enzyme in Leaves and Impairment of Photoautotrophic Growth in a C_3 Plant, Rice
• EXPRESSION OF ASPARTATE AMINOTRANSFERASE GENE OF C4 PANICUM MILIACEUMIN RICE
• THE PROMOTER OF rbcS IN A C3 PLANT (RICE) DIRECTS ORGAN-SPECIFIC, LIGHT-DEPENDENT EXPRESSION IN A C4 PLANT (MAIZE), BUT DOES NOT CONFER THE BUNDLE SHEATH CELL-SPECIFIC EXPRESSION
• FUNCTION ANALYSIS OF PYRUVATE Pi DIKINASE IN RICE
• URICASE GENE EXPRESSION IN A NON-ALLANTOIN TYPE LEGUME, MEDICAGO SATIVA
• HOW HAD THE C4-type PDK GENE ESTABLISHED DURING THE EVOLUTION FROM C3- TO C4-PLANT?
• Evolution of C4 Photosynthetic Genes and Overexpression of Maize C4 Genes in Rice
• EVOLUTION AND EXPRESSION OF the Pdk GENE IN RICE
• Engineering of C4 Photosynthesis genes in rice, a C3 plant, for increased photosynthetic efficiency
• Global Gene Expression in Bradyrhizobium japonicum Cultured with Vanillin, Vanillate, 4-Hydroxybenzoate and Protocatechuate
• NAD^+-malic enzyme affects nitrogenase activity of Mesorhizobium loti bacteroids in Lotus japonicus nodules
• Effect of Volume of Non-Surgical Embryo Transfer Medium on Ability of Porcine Embryos to Survive to Term
• NAD+-malic enzyme affects nitrogenase activity of Mesorhizobium loti bacteroids in Lotus japonicus nodules
• Differential protein profiles of Bradyrhizobium japonicum USDA110 bacteroid during soybean nodule development(Soil Biology)
• PJ-413 Eplerenone Enhances Neovascularization Induced by Endothelial Progenitor Cells in Rat Hindlimb Ischemia(Atherosclerosis, basic(07)(IHD),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
• Host plant genome overcomes the lack of a bacterial gene for symbiotic nitrogen fixation
• 8-23 Novel metallochaperone in the assembly of cytochrome c oxidase of soybean nodule bacteroids
• NAD-Malic Enzyme Affects Nitrogen Fixing Activity of Bradyrhizobium japonicum USDA 110 Bacteroids in Soybean Nodules
• 8-11 Proteome analysis of Bradyrhizobium japonicum USDA110 bacteroid differentiation during soybean nodule development
• Genetic Diversity of Native Bradyrhizobium Populations in Soybean-Growing Areas of Northern Thailand (Soil Biology)
• Symbiotic Efficiency and Compatibility of Native Rhizobia in Northern Thailand with Different Soybean Cultivars III. Laboratory Experiment Using Native Isolates from Upland Rainfed Soybean-Growing Areas
• Symbiotic Efficiency and Compatibility of Native Rhizobia in Northern Thailand with Different Soybean Cultivars II. Laboratory Experiment Using Native Isolates from Irrigated Soybean-Growing Areas
• Symbiotic Efficiency and Compatibility of Native Rhizobia in Northern Thailand with Different Soybean Cultivars I. Field Experiment in Irrigated Traditional Soybean-Growing Area
• Expression Cloning of a Variety of Novel Protein Kinases in Lotus japonicus
• Characterization of Local Rhizobia in Thailand and Distribution of Malic Enzymes
• Cloning and Characterization of a NADP^+ -Malic Enzyme Gene from Bradyrhizobium japonicum USDA110
• An Isozyme of Betaine Aldehyde Dehydrogenase in Barley
• Soybean Cotyledons Contain a Uricase That Cross-Reacts with Antibodies Raised against the Nodule Uricase (Nod-35)
• Purification and Properties of an alpha-L-Arabinofuranosidase from Cotyledons of Soybean Seedlings(Biological Chemistry)
• Cloning, Sequencing and Expression of an α-L-Arabinofuranosidase from Aspergillus sojae
• Purification and Characterization of a β-Glucosidase with β-Xylosidase Activity from Aspergillus sojae
• Purification and Characterization of an Endo-1, 4-β-D-Galactanase from Aspergillus sojae
• Succinate Degradation through the Citric Acid Cycle in Bradyrhizobium japonicum J501 Bacteroids under Low Oxygen Concentrations(Biological Chemistry)
• Proteomic Analysis on Symbiotic Differentiation of Mitochondria in Soybean Nodules
• Characterization of Bacteroid Proteins in Soybean Nodules Formed with Bradyrhizobium japonicum USDA110
• Regulation of uricase activity in developing roots of Glycine max, non-nodulating variety A62-2
• Cadaverine Involved in Urate Degrading Activity (Uricase Activity) in Soybean Radicles
• Molecular Analysis of Ribosomal RNA Gene of Red Tide Algae Obtained from the Seto Inland Sea
• Application of Repetitive Extragenic Palindromic (REP)-PCR and Enterobacterial Repetitive Intergenic Consensus (ERIC)-PCR Analysis to the Identification and Classification of Japan and Thai Local Isolates of Bradyrhizobium japonicum, Shinorhizobium melilo
• A molecular phylogenic analysis of marine red tide microalgae from the Seto Inland Sea
• A molecular phylogenic analysis of marine red tide phytoplankton
• Characteristics of a Urate-Degrading Diamine Oxidase-Peroxidase Enzyme System in Soybean Radicles
• Nucleotide Pools in Soybean Nodule Tissues, a Survey of NAD(P)/NAD(P)H Ratios and Energy Charge
• LjnsRING, a Novel RING Finger Protein, is Required for Symbiotic Interactions Between Mesorhizobium loti and Lotus japonicus
• Phosphoenolpyruvate Carboxylase Plays a Crucial Role in Limiting Nitrogen Fixation in Lotus japonicus Nodules
• Characterization of Uricase Promoter : GUS Gene Expression Using transgenic Lotus japonicus
• Characterization of Uricase Proteins Expressed Using pET32 and Soybean Nodule cDNA Clones
• Characterization of Transgenic Lotus japonicus Containing Sense and Anti-sense Uricase Genes
• Symbiotic Nitrogen Fixation at the Late Stage of Nodule Formation in Lotus japonicus and Other Legume Plants
• Expression of Two Uricase (Nodulin-35) Genes in a Non-Ureide Type Legume, Medicago sativa
• PROMOTER ANALYSIS OF URICASE II (NODULIN 35) GENES TO IDENTIFY THE ELEMENTS FOR NODULE UNINFECTED CELL-SPECIFIC EXPRESSION
• Effect of Exogenous NH^+_4-N Supply on Distribution of Ureide Content in Various Tissues of Alfalfa Plants, Medicago sativa
• 8-25 Short-term effects of ammonium sulfate application on distribution of ureide and amino acid-N in bleeding sap and underground-parts of alfalfa, Medicago sativa : Effect of exogenous N supply on ureide and amino acid-N
• Comparison of uricase II (nodulin 35) gene promoters from soybean, alfalfa and non-legume, Arabidopsis.
• PJ-518 Cardioprotective Mechanisms of Telmisartan on Cardiovascular Remodeling in Dahl Salt-Sensitive Rats(Hypertension, basic(04)(H),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
• PJ-116 Cardioprotective Effect of Tetrahydrobiopterin via Inhibiting Oxidative Stress in Failing Rat Hearts(Heart failure, basic(05)(M),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese Circulation Society)
• PJ-002 Effect of Combination Therapy with Sustained-Release bFGF and G-CSF on Angiogenesis and Cardiac Function in Myocardial Ischemia(Acute myocardial infarction, basic(02)(IHD),Poster Session(Japanese),The 72nd Annual Scientific Meeting of the Japanese
• PJ-001 Effect of Combination Therapy with Bone-Marrow Mononuclear Cell Implantation and Eplerenone on Angiogenesis and Cardiac Performance in Myocardial Ischemia(Acute myocardial infarction, basic(02)(IHD),Poster Session(Japanese),The 72nd Annual Scientif
• Preparation of a Monoclonal Antibody against Soybean Nodule Uricase (nod-35), and Immunoblot Analysis of the Expression of nod-35 in Tissues of Various Legumes
• PROMOTER ANALYSIS OF PHOSPHOENOL PYRUVATE CARBOXYKINASE GENE IN TRANSGENIC RICE
• ISOLATION AND CHARACTERIZATION OF AN URICASE II (NODULIN 35) GENE FROM ARABIDOPSIS.
• URICASE (NOD-35) GENE EXPRESSION ANALYSIS IN UREIDE AND AMIDE TYPE LEGUME PLANTS
• ANALYSES OF THE PROMOTER REGION OF THE SOYBEAN URICASE GENES USING TRANSGENIC HAIRY ROOT SYSTEM.
• 8-19 ダイズ根粒菌におけるmalic enzymeのクローニングと生理的意義の解析(8.共生)
• 8-24 大量発現系を用いたNADP-malic enzyme遺伝子発現産物の解析(8.共生)
• Cloning and Characterization of a Novel Ca^/Calmodulin-Dependent Protein Kinase I Homologue in Xenopus laevis
• Subsite Affinities of β-Glucosidase from Aspergillus sojae on Various Xylooligosaccharides
• Enzymes of purine catabolism in soybean plants
• Expression of a Gene for Uricase II (Nodulin-35) in Cotyledons of Soybean Plants
• Distribution of Glycinebetaine in Old and Young Leaf Blades of Salt-Stressed Barley Plants
• Species-Specific Probing for the Polymerase Chain Reaction Product of Ammonia Monooxygenase Gene Amplified from DNA Mixtures of Nitrosomonas europaea and Nitrosolobus multiformis
• The Modes of Action of Two Endo-1, 4-β-_D-Xylanases from Aspergillus sojae on Various Xylooligosaccharides
• Purification and Characterization of Two Xylanases and an Arabinofuranosidase from Aspergillus sojae
• FLUCTUATION OF ENZYME ACTIVITIES RELATED TO NITROGEN FIXATION, C6/Cl RATIO, AND NICOTINAMIDE NUCLEOTIDE CONTENTS DURING SOYBEAN PLANT DEVELOPMENT
• Purification and Characterization of the NADP-Malic Enzyme from Bradyrhizobium japonicum A1017
• GENE CLONING AND THE PHYSIOLOGICAL ROLE OF MALIC ENZYMES IN BRADYRHIZOBIUM JAPONICUM A1017
• ALLANTOIN PRODUCTION AND ITS UTILIZATION IN RELATION TO NODULE FORMATION IN SOYBEANS : Enzymatic Studies
• PRESENCE AND CHARACTERISTICS OF NADP-MALIC ENZYME IN SOYBEAN NODULE BACTEROIDS
• Protein Profile of Symbiotic Bacteria Mesorhizobium loti MAFF303099 in Mid-growth Phase(Biochemistry & Molecular Biology)
• ISOLATION AND CHARACTERIZATION OF A BARLEY GENE ENCODING BETAINE ALDEHYDE DEHYDROGENASE ISOZYME THAT IS NOT LOCALIZED IN MICROBODY
• Copper Metallochaperones are Required for the Assembly of Bacteroid Cytochrome c Oxidase Which is Functioning for Nitrogen Fixation in Soybean Nodules
• A Rice Mutant Sensitive to Al Toxicity is Defective in the Specification of Root Outer Cell Layers
• Transgenically Produced Glycinebetaine Protects Ribulose 1, 5-bisphosphate Carboxylase/Oxygenase from Inactivation in Synechococcus sp.PCC7942 under Salt Stress
• EFFECTS OF BETAINE ON THE PHOTO-SYNTHETIC ELECTRON TRANSPORT OF THE TRANSFORMANT-AND SALT TOLERANT-CYANOBACTERIA
• Exogenous Glycinebetaine Accumulation and Increased Salt-tolerance in Rice Seedlings
• Expression of two uricase genes in various tissues of soybean plants
• Expression and characterization of PKL01, an Ndr kinase homolog in Lotus japonicus
• 8-15 Malic enzyme distribution among local rhizobia from Thailand
• Succinate Metabolism of Isolated Soybean Nodule Bacteroids at Low Oxygen Concentration
• Involvement of a Novel Genistein-Inducible Multidrug Efflux Pump of Bradyrhizobium japonicum Early in the Interaction with Glycine max (L.) Merr
• Effects of Oxygen Concentration and Leghemoglobin on Organic Acid Degradation by Isolated Soybean Nodule Bacteriods

もっと見る 閉じる

スポンサーリンク