Novel Approach to In Vivo Screening for Radioprotective Activity in Whole Mice : In Vivo Electron Spin Resonance Study Probing the Redox Reaction of Nitroxyl
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概要
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Previously, we reported that X-irradiation enhanced the signal decay of a spin probe injected into whole mice measured by in vivo ESR, and that the observed enhancement was suppressed by the preadministration of cysteamine, a radioprotector [Miura, Y., Anzai, K., Urano, S. and Ozawa, T. (1997) Free Rad. Biol. Med. 23: 533-540]. In the present study, the suppression activity of the X-ray-induced increase in the ESR signal decay rate (termed suppression index, SI) was measured for several radioprotectors: 5-hydroxytryptamine (5-HT), S-2-(3-aminopropylamino)-ethylphosphorothioic acid (WR-2721), 4-hydroxy-2,2,6,6-tetramethyl-piperidine-N-oxyl (TEMPOL), cimetidine, interleukin-1β (IL-1β) and stem cell factor (SCF). The enhancement of the ESR signal decay of carbamoyl-PROXYL due to X-irradiation was suppressed by a treatment with all of the radioprotectors examined, showing positive SI values. However, a dose-dependency of 5-HT or WR-2721 was not observed, suggesting that several mechanisms exist for radioprotection and a modification of the signal decay rate. Although the in vivo ESR system cannot be used in place of the 30-day survival method for the assessment of radioprotectors, this system might be applicable to in vivo, non-invasive screening prior to using the 30- day survival method.
- 日本放射線影響学会の論文
著者
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Ueda Jun-ichi
Treatment Res. Team Medical Treatment For High Dose Exposure Res. Group Res. Center For Radiation Em
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OZAWA Toshihiko
Department of Health Pharmacy, Yokohama College of Pharmacy
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Miura Yuri
Tokyo Metropolitan Inst. Of Gerontology Tokyo Jpn
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Miura Yuri
Department Of Health Chemistry School Of Pharmaceutical Sciences Showa University
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Miura Yuri
Department Of Bioregulation Research National Institute Of Radiological Sciences:department Of Bioch
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Anzai Kazunori
Department Of Bioregulation Research National Institute Of Radiological Sciences
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Ueda Jun-ichi
Department Of Bioregulation Research National Institute Of Radiological Sciences
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Ueda Jun-ichi
Department Of Animal Industry Aichi Agricultural Research Center
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Ozawa Toshihiko
Department Of Bioregulation Research National Institute Of Radiological Sciences
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Ozawa T
National Inst. Radiological Sci. Chiba Jpn
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