Proteomic Analysis of Two Types of Exosomes in Human Whole Saliva
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概要
- 論文の詳細を見る
Saliva contains a large number of proteins that participate in the protection of oral tissue. Exosomes are small vesicles (30—100 nm in diameter) with an endosome-derived limiting membrane that are secreted by a diverse range of cell types. We have recently demonstrated that exosomes are present in human whole saliva. In this study, we found that whole saliva contained at least two types of exosomes (exosome I and exosome II) that are different in size and protein composition. Proteomic analysis revealed that both types of exosomes contained Alix, Tsg101 and Hsp70, all exosomal markers, immunoglobulin A and polymeric immunoglobulin receptor, whereas they had different protein compositions. Most of dipeptidyl peptidase IV known as CD26 in whole saliva, was present on the exosome II and metabolically active in cleaving chemokines (CXCL11 and CXCL12). Human whole saliva exosomes might participate in the catabolism of bioactive peptides and play a regulatory role in local immune defense in the oral cavity.
著者
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HARAZONO AKIRA
Division of Risk Assessment, Biological Safety Research Center, National Institute of Health Science
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Ogawa Yuko
Faculty of Pharmaceutical Sciences, Teikyo Heisei University
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Miura Yuri
Research Team for Mechanism of Aging, Tokyo Metropolitan Institute of Gerontology
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Kanai-Azuma Masami
Department of Anatomy, Kyorin University School of Medicine
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Akimoto Yoshihiro
Department of Anatomy, Kyorin University School of Medicine
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Kawakami Hayato
Department of Anatomy, Kyorin University School of Medicine
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Yamaguchi Teruhide
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences
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Toda Tosifusa
Research Team for Mechanism of Aging, Tokyo Metropolitan Institute of Gerontology
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Endo Tamao
Research Team for Mechanism of Aging, Tokyo Metropolitan Institute of Gerontology
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Tsubuki Masayoshi
Institute of Medicinal Chemistry, Hoshi University
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Yanoshita Ryohei
Department of Biology, Hoshi University
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Miura Yuri
Tokyo Metropolitan Inst. Of Gerontology Tokyo Jpn
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Miura Yuri
Research Team For Functional Genomics Tokyo Metropolitan Institute Of Gerontology
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Miura Yuri
Department Of Bioregulation Research National Institute Of Radiological Sciences:department Of Bioch
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Endo Tamao
Research Team For Functional Genomics Tokyo Metropolitan Institute Of Gerontology
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Kanai-azuma Masami
Department Of Anatomy Kyorin University School Of Medicine
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Yamaguchi Teruhide
Division Of Biological Chemistry And Biologicals National Institute Of Health Sciences
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Harazono Akira
Division Of Biological Evaluation National Institute Of Health Sciences Osaka Branch
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Kawakami Hayato
Department Of 2nd Anatomy Kyorin University School Of Medicine
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Tsubuki Masayoshi
Institute Of Medicinal Chemistry Hoshi University
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Akimoto Yoshihiro
Department Of Anatomy Kyorin University School Of Medicine
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Ogawa Yuko
Faculty Of Pharmaceutical Sciences Kumamoto University
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Toda Tosifusa
Research Team For Mechanism Of Aging Tokyo Metropolitan Institute Of Gerontology
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Harazono Akira
Division of Biological Chemistry and Biologicals, National Institute of Health Sciences
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Yamaguchi Teruhide
Division of Biological Chemistry & Biologicals, National Institute of Health Sciences
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