The protein expression profile of cynomolgus monkey embryonic stem cells in two-dimensional gel electrophoresis : a successful identification of multiple proteins using human databases
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概要
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Global gene and protein expression analyses have had great impacts on scientific progresses in this new era of bioinformatics. Although studies using murine and human materials can fully exploit the large volume of their databases, there are quite a few inconveniences for an investigation on non-human primate materials due to still insufficient data collections. Here we examined the availability of human databases for the protein identification process using the two-dimensional electrophoresis-based proteomic study in cynomolgus monkey embryonic stem (ES) cells. Querying public human protein databases, we successfully identified multiple protein spots via mass spectrometric analysis using MALDI-TOF apparatus. The results of the protein identification were confirmed by western blotting using polyclonal antibodies raised against human epitopes. Interestingly, the results of western blotting further identified the existence of previously unreported multiple isoforms of common proteins including glycolytic pathway enzymes. Thus, combined analyses of the mass spectrometry querying the Homo sapience databases and the western blotting using polyclonal antibodies is highly effective in determining protein expressions in monkey cells. Our success in obtaining a draft protein expression profile of cynomolgus monkey ES cells will contribute to the promotion of non-human primate ES cell researches.
- 2007-03-01
著者
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Kanegasaki Shiro
宮崎医科大学 小児科
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Kanegasaki S
Section Of Bacterial Infection Institute Of Medical Science The University Of Tokyo
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Kanegasaki Shiro
国立国際医療センター
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Kanegasaki Shiro
Institute Of Medical Science The University Of Tokyo
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NAKAMURA Naoko
Department of Pathology, Institute of Basic Medical Sciences, Institute of Clinical Medicine, Univer
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Yasuda Kazuki
Advanced Medical Research Laboratories Tanabe Seiyaku Co. Ltd.
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Toda Tosifusa
Research Team for Mechanism of Aging, Tokyo Metropolitan Institute of Gerontology
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Toda Tosifusa
Research Team For Molecular Biomarkers Tokyo Metropolitan Institute Of Gerontology
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Toda Tosifusa
東京都老人総合研究所 老化ゲノムバイオマーカー研究チーム
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Kondo Yasushi
Advanced Medical Research Laboratories, Tanabe Seiyaku Co., Ltd
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NAKAMURA Megumi
Research Team for Molecular Biomarkers, Proteomic Gerontology Research Unit, Tokyo Metropolitan Inst
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KIMURA Akiko
Department of Materials Physics, Osaka University
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SAEKI Koichi
Department of Electronic Engineering,Tohoku University
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Kondo Yasushi
Advanced Medical Research Laboratories Tanabe Seiyaku Co. Ltd
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Kamisaka Yasushi
Advanced Medical Research Laboratories Tanabe Seiyaku Co. Ltd.
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Nakamura Naoko
Department Of Hematology Research Institute International Medical Center Of Japan
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KABURAGI YASUSHI
Department of Metabolic Disorder, Research Institute, International Medical Center of Japan
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NAKAHARA Masako
Department of Hematology, Research Institute, International Medical Center of Japan
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SAEKI Kumiko
Department of Hematology, Research Institute, International Medical Center of Japan
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YOGIASHI Yoshiko
Department of Hematology, Research Institute, International Medical Center of Japan
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HORIUCHI Akiko
Department of Hematology, Research Institute, International Medical Center of Japan
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YONEDA Asako
Department of Hematology, Research Institute, International Medical Center of Japan
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MATSUYAMA Satoko
Department of Hematology, Research Institute, International Medical Center of Japan
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YUO Akira
Department of Hematology, Research Institute, International Medical Center of Japan
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NAKATSU Masami
Department of Hematology, Research Institute, International Medical Center of Japan
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Yoneda Asako
Department Of Hematology Research Institute International Medical Center Of Japan
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Horiuchi Akiko
Department Of Hematology Research Institute International Medical Center Of Japan
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Matsuyama Satoko
Department Of Hematology Research Institute International Medical Center Of Japan
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Yogiashi Yoshiko
Department Of Hematology Research Institute International Medical Center Of Japan
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Nakahara Masako
Department Of Hematology Research Institute International Medical Center Of Japan
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Saeki Koichi
Department Of Electrical Engineering Faculty Of Engineering Science Osaka University:(present Addres
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Kimura Akiko
Department Of Hematology Research Institute International Medical Center Of Japan
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Yuo Akira
Department Of Hematology Research Institute International Medical Center Of Japan
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Saeki Kumiko
Department Of Hematology Research Institute International Medical Center Of Japan
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Toda Tosifusa
Research Team For Mechanism Of Aging Tokyo Metropolitan Institute Of Gerontology
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Nakatsu Masami
Department Of Hematology Research Institute International Medical Center Of Japan
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Nakamura Megumi
Research Team For Mechanism Of Aging Tokyo Metropolitan Institute Of Gerontology
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Nakamura Naoko
Department Of Bioscience And Biotechnology Graduate School Of Natural Science And Technology Okayama
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Kaburagi Yasushi
Department Of Internal Medicine And Molecular Science Graduate School Of Medicine Osaka University
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Kaburagi Yasushi
Advanced Medical Research Laboratories Tanabe Seiyaku Co. Ltd.
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Kaburagi Yasushi
Department Of Diabetic Complications Diabetes Research Center Research Institute National Center For Global Health And Medicine
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