Molecular Cloning and Expression of Starfish Protein Kinase C Isoforms
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概要
- 論文の詳細を見る
To investigate the roles of protein kinase C (PKC) isoforms in Echinoderms, we cloned starfish cDNAs for novel, atypical, and conventional PKCs. They showed highest homology with PKCδ, ι, and α isoforms respectively. It was predicted from the whole genome sequence and by RT-PCR that sea urchin has only one isoform of each PKC subgroups. It is thus likely that these isoforms are the prototypes or ancestors of the PKC subgroups. The phylogenetic tree suggests that atypical PKC was first formed by evolution from the common prototype of AGC protein kinase family, and novel and conventional PKCs next. RT-PCR analysis indicated that novel and atypical PKC mRNAs are expressed ubiquitously in all tissues of adult starfish, whereas conventional PKC mRNA is expressed mainly in the ovary and oocytes, and only slightly in the tube foot and stomach. Upon heterologous expression, only atypical PKC was expressed in the functional form in insect cells.
著者
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NAKAMURA Naoko
Department of Pathology, Institute of Basic Medical Sciences, Institute of Clinical Medicine, Univer
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Toraya Tetsuo
Department Of Bioscience And Biotechnology Graduate School Of Natural Science And Technology Okayama
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MIYAKE Yasuo
Department of Bioscience and Biotechnology, Faculty of Engineering, Okayama University
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KONDO Tsunenori
Department of Urology, Tokyo Women's Medical University
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IKEDA KAORI
Department of Internal Medicine, Kobe City General Hospital
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Miyake Yasuo
Department Of Bioscience And Biotechnology Graduate School Of Natural Science And Technology Okayama
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YASUI Masaru
Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okaya
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TSUKAMOTO Shinpei
Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okaya
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HORI Chinami
Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okaya
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OKEMOTO Naomi
Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okaya
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MASHOU Keiko
Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okaya
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BANDO Reiko
Department of Bioscience and Biotechnology, Graduate School of Natural Science and Technology, Okaya
関連論文
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