Cloning, Sequencing, and Heterologous Expression of Rat Methionine Synthase cDNA
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概要
- 論文の詳細を見る
Methionine synthase catalyzes cobalamin-dependent methyl transfer reaction from 5-methyltetrahydrofolate to homocysteine, forming methionine. Rat methionine syn-thase cDNA was cloned and analyzed by RT-PCR, 3'- and 5'-RACE techniques. The cDNA consists of a 0.3-kb up-stream untranslated region, a 3.8-kb coding region, and a 0.4-kb downstrean untranslated region. The open reading frame encoded a polypeptide of 1,253 amino acid residues with a calculated molecular weight of 139,162. This molecu-lar weight was in good agreement with the observed one (143,000) of the purified rat liver enzyme. The deduced amino acid sequence was 53,92,and 64% identical with those of the Escherichia coli, human, and presumptive Caenorhabditis elegans enzymes, respectively. All the fingerprint sequences, forming parts of the cobalamin- and S-adenosylmethionine-binding sites, were completely conserved in the rat methionine synthase. A high-level expression of catalytically active enzyme in insect cells was done by infection with a baculovirus containing the rat methionine synthase cDNA.
- 社団法人日本農芸化学会の論文
- 1998-11-23
著者
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Yamada Kazuhiro
Department of Hard Tissue Research, Matsumoto Dental University Graduate School of Oral Medicine
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Tobimatsu T
Faculty Of Engineering Okayama University
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Tobimatsu Takamasa
Department Of Bioscience And Biotechnology Faculty Of Engineering Okayama University
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Toraya Tetsuo
Department Of Bioscience And Biotechnology Graduate School Of Natural Science And Technology Okayama
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Toraya Tetsuo
Department Of Bioscience And Biotechnology Faculty Of Engineering Okayama University
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Toraya Tetsuo
Faculty Of Engineering Okayama University
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Yamashita Kenji
Biochemical Research Laboratories Kanegafuchi Chemical Industry Co. Ltd.
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Yamada K
Faculty Of Engineering Okayama University:(present Address)department Of Biotechnology And Life Scie
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Yamada Kazuhiro
Department Of Gastroenterology And Metabolism Nagoya City University Graduate School Of Medical Scie
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Katayama Y
Graduate School Of Bio-applications And Systems Engineering Tokyo University Of Agriculture And Tech
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Yamada Kazuhiro
Department Of Applied Physics And Physico-informatics Faculty Of Science And Technology Keio University
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YAMADA Kazuhiro
Department of Agricultural Chemistry, Tokyo University of Agriculture
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