Comparison of Human Lymphotoxin Gene Expression in CHO Cells Directed by Genomic DNA or cDNA Sequences(Biological Chemistry)
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概要
- 論文の詳細を見る
Four recombinant plasmids coding for human lymphotoxin (LT) were constructed with genomic DNA (gDNA) or cDNA sequences. The simian virus 40 (SV40) early region, which contains the early promoter, an intron of the small-t-antigen-encoding gene, and polyadenylation signal sequences, was used for transcriptional and post-transcriptional regulatory elements in the construction of these plasmids. Two of them contained gDNA and the other two contained cDNA. One of the gDNA plasmids and one of the cDNA plasmids carry the SV40 intron between the structural gene and polyadenylation signal. Transient and stable gene expression levels of these plasmids in Chinese hamster ovary (CHO) cells were measured by assaying the secreted LT. The plasmid carrying gDNA without the SV40 intron was expressed more efficiently than the other three plasmids in both transient and stable gene expression assays.
- 社団法人日本農芸化学会の論文
- 1990-11-23
著者
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Ikenaka Y
Fine Chemicals Research Laboratories Kaneka Corporation
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Yamashita Kenji
Biochemical Research Laboratories Kanegafuchi Chemical Industry Co. Ltd.
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Kakutani Tetsu
Biochemical Research Laboratories Kanegafuchi Chemical Industry Co. Ltd.
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Ikenaka Yasuhiro
Biochemical Research Laboratories Kanegafuchi Chemical Industry Co. Ltd.
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KAWAHARADA Hajime
Biochemical Research Laboratories, Kanegafuchi Chemical Industry Co., Ltd.
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WATANABE Kiyoshi
Biochemical Research Laboratories, Kanegafuchi Chemical Industry Co., Ltd.
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Watanabe Kiyoshi
Biochemical Research Laboratories Kanegafuchi Chemical Industry Co. Ltd.
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Yajima Kazuyoshi
Fine Chemicals Research Laboratories Kaneka Corporation
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Yajima Kazuyoshi
Sano College Sano Nihon University Gakuen
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Yamashita K
Tohoku Univ. Sendai Jpn
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Watanabe Kazuyoshi
Faculty Of Pharmaceutical Sciences Toyama Medical And Pharmaceutical University:institute Of Cytosig
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Kajikawa Tamio
Biochemical Research Laboratories Kanegafuchi Chemical Industry Co. Ltd.
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Watanabe K
Kureha Biomedical Research Lab. Tokyo Jpn
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Kawaharada H
Biochemical Research Laboratories Kanegafuchi Chemical Industry Co. Ltd.
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Kawaharada Hajime
Biochemical Research Laboratories Kanegafuchi Chemical Industry Co. Ltd.
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Watanabe Koju
Kureha Biomedical Research Laboratories:department Of Orthopedic Surgery National Defense Medical Co
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