MONITORING OF GENE EXPRESSION IN DIFFERENTIATION OF EMBRYOID BODIES FROM CYNOMOLGUS MONKEY EMBRYONIC STEM CELLS IN THE PRESENCE OF BISPHENOL A
スポンサーリンク
概要
- 論文の詳細を見る
An embryonic stem (ES) cell differentiation model would facilitate analysis of developmental processes at the cellular level and the effects of embryotoxic and teratogenic factors in vitro. We explored the use of differentiation of embryoid bodies (EBs) from cynomolgus monkey ES cells for embryotoxicity testing. We determined the mRNA expression of various genes using real-time RT-PCR. Oct-3/4 expression was almost completely suppressed on day 14, suggesting that ES cells reached differentiated status in around 14 days. mRNA expression of E-cadherin, connexin 43, caveolin-1, and argininosuccinate synthetase was reproducibly suppressed during EB differentiation in 7-32% of ES cells in three separate experiments. Although these may not be "general sternness marker genes" such as Oct-3/ 4, they could play a role in readying stem cells for differentiation in response to deletion of signals from feeder cells. Next, we examined the effects of bisphenol A (BPA) on the mRNA expression of several differentiation marker genes for ES cells. That of PAX-6, an ectoderm marker, with 0, 0.1, and 10 μM BPA in 21-day EBs was 3,500%, 6,668%, and 8,394%, respectively, compared with ES cells. The difference between doses of 0 and 10 μM BPA in 21-day EBs was statistically significant (p=0.049). Pax-6 activation in the presence of BPA may interfere with the development of eyes, sensory organs, and certain neural and epidermal tissues usually derived from ectodermal tissues. Differentiation of EBs from cynomolgus monkey ES cells could be a useful model for detecting gene expression changes in response to chemical exposure.
- 日本トキシコロジー学会の論文
- 2007-08-17
著者
-
AKIBA Suminori
Department of Epidemiology and Preventive Medicine, Kagoshima University, Graduate School of Medical
-
Terao Keiji
Tsukuba Primate Center, National Institute of Infectious Diseases
-
Kondo Yasushi
Advanced Medical Research Laboratories, Tanabe Seiyaku Co., Ltd
-
Yamamoto Megumi
Physiology Section Department Of Basic Medical Sciences National Institute For Minamata Disease:envi
-
Akiba Suminori
鹿児島大学 大学院医歯学総合研究科疫学･予防医学
-
Akiba Suminori
Department Of Epidemiology And Preventive Medicine Kagoshima University Graduate School Of Medical A
-
Tase Naomi
Tsukuba Primate Research Center National Institute Of Biomedical Innovation
-
Kondo Yasushi
Advanced Medical Research Laboratories Tanabe Seiyaku Co. Ltd
-
Kondo Yasushi
Advanced Medical Research Laboratories Tanabe Seiyaku Co. Ltd.
-
Terao Keiji
Tsukuba Primate Res. Center National Inst. Of Biomedical Innovation
-
Terao Keiji
Tsukuba Primate Research Center National Institute Of Biomedical Innovation
-
Terao Keiji
Tsukuba Primate Center For Medi.sci. Nation.inst.health
-
Okuno Tsuyoshi
Advanced Medical Research Laboratories Tanabe Seiyaku Co. Ltd.
-
SHIMOZAWA Nobuhiro
Tsukuba Primate Research Center, National Institute of Biomedical Innovation
-
Shimozawa Nobuhiro
Tsukuba Primate Research Center National Institute Of Biomedical Innovation
-
Terao Keiji
National Inst. Of Biomedical Innovation Tsukuba
関連論文
- Lifestyle Factors and Visible Skin Aging in a Population of Japanese Elders
- Expression of Thymidine Phosphorylase in Human Gastric Carcinoma
- PJ-369 Few Transplanted Bone Marrow CD34^+ Cells Become Endothelial and Myocardial Cells in Ischemic Myocardium in Nonhuman Primates(Angiogenesis 3 (IHD) : PJ62)(Poster Session (Japanese))
- Molecular Evolution Inferred from Immunological Cross-reactivity of Immunoglobulin G among Chiroptera and Closely Related Species
- Epstein-Barr Virus-associated Gastric Carcinoma in Japanese Brazilians and Non-Japanese Brazilians in Sao Paulo
- Identification of Vascular Progenitor Cells from Human Embryonic Stem Cells and their Application to Vascular Regeneration Therapy(The 69th Annual Scientific Meeting of the Japanese Circulation Society)
- Identification of 'Vascular Progenitor Cells' from Primate Embryonic Stem Cells
- HTLV-1 provirus load in peripheral blood lymphocytes of HTLV-1 carriers is diminished by green tea drinking
- Safe And Efficient Collection of Cytokine-Mobilized Peripheral Blood Cells From Cynomolgus Monkeys (Macaca fascicularis) with Human Newborn-Equivalent Body Weights
- Infection of an HIV-1/SIVmac Chimeric Virus Having HIV-1 env to Macaque Monkeys via the Vaginal Cavity
- Logistic Model Analysis of Neurological Findings in Minamata Disease and the Predicting Index
- Ultraviolet Radiation and Health : from Hazard Identification to Effective Prevention
- Mortality Risks of Oesophageal Cancer Associated with Hot Tea, Alcohol, Tobacco and Diet in Japan
- Cancer Mortality in Minamata Disease Patients Exposed to Methylmercury Through Fish Diet
- Obesity affects expression of progesterone receptors and node metastasis of mammary carcinomas in postmenopausal women without a family history
- Circadian Rhythm in Circulating CD16-positive Natural Killer (NK) Cells in Macaque Monkeys, Implication of Plasma Cortisol Levels
- Relationship Between Ambient Sulfur Dioxide Levels and Neonatal Mortality Near the Mt. Sakurajima Volcano in Japan
- INVESTIGATION OF THE MECHANISM OF DRUG-INDUCED AUTOIMMUNE HEMOLYTIC ANEMIA IN CYNOMOLGUS MONKEYS ELICITED BY A REPEATED-DOSE OF A HUMANIZED MONOCLONAL ANTIBODY DRUG
- The protein expression profile of cynomolgus monkey embryonic stem cells in two-dimensional gel electrophoresis : a successful identification of multiple proteins using human databases
- Distribution of U5 Antigen on Lymphocyte Subsets in Human and Nonhuman Primates
- Chromosome Sorting of Primates : Isolation and Identification of Y Chromosome in Green Monkeys
- Possible Role of Genetic Factor(s) on Age-related Increase of Peripheral CD4^+CD^+8 Double Positive T Cells in Cynomolgus Monkeys
- Peripheral blood extrathymic CD4^+CD8^+ T cells with high cytotoxic activity are from the same lineage as CD^+CD8^- T cells in cynomolgus monkeys
- Age-Related Changes in Major Lymphocyte Subsets in Cynomolgus Monkeys
- Comparative Analysis of Human and Macaque Monkey CD4: Differences in Formaldehyde Lability and Conformation
- Peripheral blood CD4^+CD8^+ lymphocytes in cynomolgus monkeys are of resting memory T lineage
- Asthma-Like Disease in the Children Living in the Neighborhood of Mt. Sakurajima
- Upgrading of Flow Cytometric Analysis for Absolute Counts, Cytokines and Other Antigenic Molecules of Cynomolgus Monkeys (Macaca fascicularis) by Using Anti-Human Cross-Reactive Antibodies
- COMPARISON BETWEEN DENSITY GRADIENT CENTRIFUGAL METHOD AND WHOLE BLOOD METHOD FOR LYMPHOCYTE SURFACE MARKER ANALYSIS WITH FLOW-CYTOMETRY IN RHESUS MONKEYS
- Establishment of Two Substrains, Diabetes-Prone and Non-Diabetic, from Long-Evans Tokushima Lean (LETL) Rats
- MONITORING OF GENE EXPRESSION IN DIFFERENTIATION OF EMBRYOID BODIES FROM CYNOMOLGUS MONKEY EMBRYONIC STEM CELLS IN THE PRESENCE OF BISPHENOL A
- Essentials for starting a pediatric clinical study (3) : Dynamic changes in early development of immune system in macaque monkeys : The significance from standpoint of preclinical toxicity test using nonhuman primates
- Strategies for Tolerance Induction against Xenogeneic-Gene-Transduced Hematopoietic Stem Cells and Their Selective Expansion in a Monkey Model
- STANDARDIZATION OF DENSITY GRADIENT CENTRIFUGAL METHOD FOR LYMPHOCYTE SURFACE MARKER ANALYSIS WITH FLOW-CYTOMETRY IN CYNOMOLGUS MONKEYS.
- Distribution of Lead and Endotoxin-Detoxicating Activity of Tissue Extracts in Lead-treated Mice
- Behavioral evidence of color vision deficiency in a protanomalia chimpanzee (Pan troglodytes)
- Polymorphic variations in long- and middle-wavelength-sensitive opsin gene loci in crab-eating monkeys
- The Repeated Procedure of Weaning and Peer Group Formation Causes Accumulation of Stress and Changes of Plasma Cortisol Level and Natural Killer Activity in Squirrel Monkeys (Saimiri sciureus)
- Familial early onset macular degeneration in cynomolgus monkeys (Macaca fascicularis)
- Cancer Mortality in the High Background Radiation Areas of Yangjiang, China during the Period between 1979 and 1995
- A Case-control Study of Nasopharyngeal Carcinoma in the High Background Radiation Areas of Yangjiang, China
- Excess Relative Risk of Solid Cancer Mortality after Prolonged Exposure to Naturally Occurring High Background Radiation in Yangjiang, China
- The Sex Differences in Cord-Blood Cholesterol and Fatty-Acid Levels Among Japanese Fetuses
- Endotoxin Susceptibility of Mice Treated with Lead Acetate
- Possible Protective Effect of Milk, Meat and Fish for Cerebrovascular Disease Mortality in Japan
- Simple Method for Assaying Serum Oxytocin and Changes of Serum Oxytocin Level during Parturition in Cynomolgus Monkeys
- Toenail selenium levels and gastric cancer risk in Cali, Colombia
- Possible Role of Peripheral CD14^ Monocytes in the Development of Collagen-Induced Arthritis in Cynomolgus Monkeys (Macaca fascicularis)
- Non-human Primate Model in New Field of Immunology, Psychoneuro-Immunology : Significance for Assessment of Environment Enrichment and Psychological Well-being
- White matter activated glial cells produce BDNF in a stroke model of monkeys
- Collection and culture of primordial germ cells from cynomolgus monkeys (Macaca fascicularis)
- Molecular Evolution of lgG Subclass among Nonhuman Primates : Implication of Differences in Antigenic Determinants among Apes
- Development of a microsatellite marker set applicable to genome-wide screening of cynomolgus monkeys (Macaca fascicularis)
- Interactive Effects between Radiation and Other Factors on Cancer Risk among A-Bomb Survivors - an Overview of RERF Studies : II. BIOLOGICAL EFFECTS
- Epstein-Barr virus associated gastric carcinoma : Epidemiological and clinicopathological features