Comparative Analysis of Human and Macaque Monkey CD4: Differences in Formaldehyde Lability and Conformation
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概要
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Here we characterized macaque monkey CD4 by flow cytometry. The results showed that relatively lower fluorescence intensity was observed depending on the monoclonal antibodies (mAbs) used for staining; Leu-3a exhibited four-fold lower intensity than Nu-Th/i, and that formaldehyde fixation dramatically reduced fluorescence intensity of macaque CD4+ cells stained with Leu-3a but not of human cells. Nu-Th/i is therefore preferable for the analysis of macaque CD4. Pretreatment of either mAb inhibited the other mAb binding to human CD4. On the contrary, Nu-Th/i inhibited Leu-3a binding but Leu-3a poorly blocked Nu-Th/i binding to the macaque CD4. These results indicate that Leu-3a and Nu-Th/i epitopes are conserved in macaque CD4 but Leu-3a epitope is conformationally cryptic and/or fragile, resulting in the lower affinity. Amino acid sequence alignment of CD4 domain 1 shows that the substitutions outside the linear Leu-3a epitope may determine these characteristics of macaque CD4.
- 社団法人 日本実験動物学会の論文
著者
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Akari Hirofumi
Tsukuba Primate Research Center, National Institute of Biomedical Innovation
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Terao Keiji
Tsukuba Primate Center, National Institute of Infectious Diseases
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YOSHIKAWA Yasuhiro
Tsukuba Primate Center, National Institute of Infectious Diseases
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Yoshikawa Y
Department Of Biomedical Science The Graduate School Of Agricultural And Life Sciences The Universit
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Akari H
Department Of Virology School Of Medicine University Of Tokushima
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Akari H
徳島大学医学部
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ADACHI Akio
Department of Virology, Tokushima University School of Medicine
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Nam Ki-hoan
Tsukuba Primate Center National Institute Of Infectious Diseases
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Nam Ki-hoan
Iclas Monitoring Subcenter Korea Korea Res. Inst. Of Bioscience And Biotechnology
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Akari Hirofumi
Tsukuba Primate Center For Medical Science National Institute Of Health
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Yoshikawa Yasuhiro
Department Of Biomedical Science The Graduate School Of Agricultural And Life Sciences The Universit
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Terao K
National Inst. Infectious Diseases Ibaraki Jpn
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Terao Keiji
Tsukuba Primate Center National Institute Of Infectious Diseases
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Terao Keiji
Tsukuba Primate Center For Medi.sci. Nation.inst.health
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Adachi Akio
Department Of Virology School Of Medicine The University Of Tokushima
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Yoshikawa Yasuhiro
Tsukuba Primate Center
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Terao K
Tsukuba Primate Center National Institute Of Infectious Diseases
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Terao Kenichi
Department Of Microbiology And Immunology Tokyo Metropolitan Institute For Neuroscience (tmin)
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