Epitope Mapping of Murine Monoclonal Antibodies against Human Immunodeficiency Virus Type 1 Nef
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概要
- 論文の詳細を見る
It has shown that the human immunodeficiency virus type 1 (HIV-1) Nef protein has the high antigenicity in HIV-1 seropositive individuals. We newly obtained seven monoclonal antibodies (mAbs). To identify the antigenic determinants of HIV-1 Nef protein against murine, epitope mapping of the mAbs was performed by enzyme-linked immunosorbent assay (ELISA) by using several recombinant truncated Nef fusion proteins, that were expressed in Esherichia coli, and synthetic peptides. The results showed that mAbs A6, A7, F2, F3, F4, F8 and E5 recognized epitopes on Nef protein located at amino acid residues 18-26, 28-45, 115-137, 128-137, 115-126, 128-137, and 170-181, respectively.
- 社団法人 日本実験動物学会の論文
著者
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Kitoh Junzoh
Institute For Laboratory Animal Research Nagoya University School Of Medicine
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Tashiro Masato
Department Of Virology National Institute Of Infectious Diseases
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Tashiro Masato
Department Of Viral Disease And Vaccine Control National Institute Of Infectious Disease
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Tashiro Masato
Department Of Virology 1 National Institute Of Health
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OTAKE Kaori
Institute for Laboratory Animal Research, Nagoya University School of Medicine
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FUJII Yoichi
Institute for Laboratory Animal Research, Nagoya University School of Medicine
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ADACHI Akio
Department of Virology, Tokushima University School of Medicine
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Fujii Yoichi
Institute For Laboratory Animal Research Nagoya S ' University School Of Medicine
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Kitoh Junzoh
Institute For Experimental Animal Research Nagoya University School Of Medicine
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Fujii Y
Hamamatsu University School Of Medicine
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Fujii Yuji
Iwakuni Research Laboratory Nippon Paper Industries Co. Ltd.
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Adachi Akio
Department Of Virology School Of Medicine The University Of Tokushima
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Adachi Akio
Department Of Virology Tokushima University School Of Medicine
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Otake Kaori
Institute For Laboratory Animal Research Nagoya University School Of Medicine
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Fujii Y
Hamamatsu Univ. School Of Medicine Hamamatsu Jpn
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KITOH Junzoh
Institute for Laboratory Animal Research, Nagoya University School of Medicine
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Tashiro Masato
Department of Molecular Microbiology and Immunology, Nagasaki University Graduate School of Biomedical Sciences
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