Comparison of the Effects of Two Fixatives for Immunolocalization of Testosterone in the Testes of the Cynomolgus Monkey, Mouse and Rat
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概要
- 論文の詳細を見る
We compared the effect of two fixatives, Bouins fixative and neutralized buffered 4% formaldehyde (10% formalin), for immunolocalization of testosterone in the testes of cynomolgus monkeys, mice and rats. In the samples fixed with Bouins fixative, immunoreactive testosterone was detected as intense deposits in the cytoplasm of Leydig cells of monkeys and mice. Immunoreactive testosterone was detected not only in Leydig cells of rats but also moderately shown within tubules. Immunoreactive testosterone could not be detected in the testes of monkeys, mice or rats fixed with neutralized buffered formalin because of the poor morphology caused by the fixative. It is concluded that Bouins fixative is a suitable fixative for immunolocalization of testosterone in the testes of cynomolgus monkeys, mice and rats.
- 社団法人 日本実験動物学会の論文
- 2000-10-01
著者
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Yoshikawa Y
Univ. Tokyo Tokyo Jpn
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YOSHIDA Takashi
Tsukuba Primte Center, National Institute of Infections Diseases
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LIANG Ji-Hong
Tsukuba Primate Center, National Institute of Infectious Diseases
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SANKAI Tadashi
Tsukuba Primate Center, National Institute of Infectious Diseases
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YOSHIKAWA Yasuhiro
Tsukuba Primate Center, National Institute of Infectious Diseases
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Yoshikawa Y
Univ. Tokyo Tokyo
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Yoshida T
Nagoya Univ. Nagoya Jpn
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Yoshida T
Matsushita Electric Co. Tochigi Jpn
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Liang Ji-hong
Tsukuba Primate Center National Institute Of Infectious Diseases
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Sankai T
Tsukuba Primate Center For Medical Science National Institute Of Infectious Diseases
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Sankai T
Tsukuba Primate Research Center National Institute Of Biomedical Innovation
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Sankai Tadashi
Tsukuba Primate Center For Medical Science National Institute Of Infectious Diseases
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Yoshida Takashi
Tsukuba Primate Center For Medical Science National Institute Of Infectious Diseases
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Yoshikawa Yasuhiro
Tsukuba Primate Center
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