Single-Step Generation of Rabbits Carrying a Targeted Allele of the Tyrosinase Gene Using CRISPR/Cas9
スポンサーリンク
概要
- 論文の詳細を見る
Targeted genome editing of nonrodent mammalian species has provided the potential for highly accurate interventions into gene function in humans and the generation of useful animal models of human diseases. Here we show successful clustered regularly interspaced short palindromic repeat (CRISPR) and CRISPR-associated (Cas)-mediated gene targeting via circular plasmid injection in rabbits. The rabbit tyrosinase gene (TYR) was effectively disrupted, and we confirmed germline transmission by pronuclear injection of a circular plasmid expressing humanized Cas9 (hCas9) and single-guide RNA. Direct injection into pronuclear stage zygotes was possible following an in vitro validation assay. Neither off-target mutagenesis nor hCas9 transgenesis was detected in any of the genetically targeted pups and embryos examined. Gene targeting with this rapid and simplified strategy will help accelerate the development of translational research using other nonrodent mammalian species.
- Japanese Association for Laboratory Animal Scienceの論文
著者
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Ogura Atsuo
Riken Bioresource Center
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IKAWA Masahito
Research Institute for Microbial Diseases
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Sankai Tadashi
Tsukuba Primate Center For Medical Science National Institute Of Infectious Diseases
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HIROSE Michiko
RIKEN BioResource Center, Ibaraki 305-0074, Japan
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Iguchi Atsushi
Interdisciplinary Research Organization, University of Miyazaki
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HONDA Arata
Organization for Promotion of Tenure Track, University of Miyazaki
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YASMIN Lubna
Tsukuba Primate Research Center, National Institute of Biomedical Innovation
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YUZAWA Kazuaki
Tsukuba Primate Research Center, National Institute of Biomedical Innovation
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HONSHO Kimiko
Organization for Promotion of Tenure Track, University of Miyazaki
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IZU Haruna
Organization for Promotion of Tenure Track, University of Miyazaki
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- Preface
- Preface
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- Single-Step Generation of Rabbits Carrying a Targeted Allele of the Tyrosinase Gene Using CRISPR/Cas9