Short-Term Preservation of Mouse Oocytes at 5℃
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概要
- 論文の詳細を見る
The temporary preservation of oocytes without freezing would be useful for some experiments. ICR mouse oocytes were kept in a preservation medium under mineral oil for 1, 2, 3, 4 or 7 days at 5°C, and 1 or 2 days at 37°C. In vitro fertilization was attempted on oocytes rinsed with TYH medium after preservation. More than 70% of morphologically normal oocytes were recovered from each preservation group. Fertilization rates of oocytes preserved for 1, 2, 3, 4 or 7 days at 5°C were 69.9, 66.5, 45.3, 26.7 and 8.8% respectively. Fertilization rates of oocytes preserved for 1 or 2 days at 37°C were 9.6 and 1.6%, respectively. Preservation of oocytes at 5°C has some capability as a method of short-term storage without freezing.
- 社団法人 日本実験動物学会の論文
- 2001-10-01
著者
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KANAYAMA Kiichi
Department of Veterinary physiology, Nihon University School of Veterinary Medicine
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SANKAI Tadashi
Tsukuba Primate Center, National Institute of Infectious Diseases
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Ogonuki Narumi
Tsukuba Primate Center National Institute Of Infectious Diseases
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Kanayama Kiichi
Department Of Veterinary Medicine College Of Bioresource Sciences Nihon University
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Sankai T
Tsukuba Primate Center For Medical Science National Institute Of Infectious Diseases
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Sankai T
Tsukuba Primate Research Center National Institute Of Biomedical Innovation
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Sankai Tadashi
Tsukuba Primate Center For Medical Science National Institute Of Infectious Diseases
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Kuwana Takashi
National Institute For Minamata Disease
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Tsuchiya Hideaki
Research Center For Animal Life Science Shiga University Of Medical Science
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TSUCHIYA Hideaki
Tsukuba Primate Center, National Institute of Infectious Diseases
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Kuwana T
Laboratory Of Intellectual Fundamentals For Environmental Studies National Institute For Environment
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Kanayama Kiichi
Department Of Veterinary Medicine College Of Bigresorce Sciences Nihon University
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