Zona-Float Method for Separating Mouse Eggs from Other Cells
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概要
- 論文の詳細を見る
We have developed a new method for separating mouse eggs from other cells, such as cumulus cells, using centrifugation with Percoll. Solutions of 45, 22.5, 11.3, and 5.6% Percoll were tested. With the 22.5% solution, 99% of whole eggs obtained by in vitro fertilization were collected from the upper part of the Percoll solution, and 98% of 2-cell embryos collected from these eggs developed to the blastocyst stage. Offspring were obtained after transfer of collected embryos to female mice. The greatest advantage of this method is that undamaged eggs are separated from other cells in one simple operation, regardless of the number of eggs.
- 社団法人 日本実験動物学会の論文
著者
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Sankai T
Tsukuba Primate Center For Medical Science National Institute Of Infectious Diseases
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Sankai T
Tsukuba Primate Research Center National Institute Of Biomedical Innovation
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Sankai Tadashi
Tsukuba Primate Center For Medical Science National Institute Of Infectious Diseases
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Hirose Yoshihiro
Tsukuba Primate Center For Medical Science National Institute Of Infectious Diseases
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MANONMANI PERIYASAMY
Tsukuba Primate Center for Medical Science, National Institute of Infectious Diseases
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Manonmani Periyasamy
Tsukuba Primate Center For Medical Science National Institute Of Infectious Diseases
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Okada Hironori
Tsukuba Primate Center For Medical Science National Institute Of Infectious Diseases
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ITO Masao
Tokyo University of Agriculture
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- Preface
- Collection and culture of primordial germ cells from cynomolgus monkeys (Macaca fascicularis)
- Fertilization and preimplantation development of mouse oocytes after prolonged incubation with caffeine
- Zona-Float Method for Separating Mouse Eggs from Other Cells
- In Vitro Manipulation of Nonhuman Primate Gametes for Embryo Production and Embryo Transfer
- Live Offspring from Cryopreserved Embryos Following In Vitro Growth, Maturation and Fertilization of Oocytes Derived from Preantral Follicles in Mice
- Single-Step Generation of Rabbits Carrying a Targeted Allele of the Tyrosinase Gene Using CRISPR/Cas9