Genetic Influences in Mouse Spermatogonial Stem Cell Self-Renewal
スポンサーリンク
概要
- 論文の詳細を見る
Spermatogonial stem cells (SSCs) are slowly dividing cells that undergo self-renewal division to support spermatogenesis. Although the effects of genetic background in stem cell self-renewal have been well studied in hematopoietic stem cells, little is known about its effect on stem cells in other self-renewing tissues, including SSCs. To examine whether genetic factors are involved in regulation of SSC self-renewal, we first studied spermatogenesis in different inbred mouse strains (C57BL/6, DBA/2, AKR, BALB/C and C3H) after chemical damage caused by busulfan. Spermatogenesis in the DBA/2 and AKR strains was relatively resistant to busulfan treatment, whereas spermatogenesis was diminished in C57BL/6 mice and nearly ablated in C3H and BALB/C mice. Serial germ cell transplantation experiments provided functional evidence that SSCs with the DBA/2 background expanded more rapidly than those with the B6 background. Finally, we also employed the Germline Stem (GS) cell culture technique to examine the self-renewal activity in vitro. Although genetic manipulation of GS cells has been limited to those from the DBA/2 background, we produced transgenic offspring of the C3H background by electroporation of GS cells with a plasmid vector. Our results underscore the importance of genetic factors in SSC self-renewal. Furthermore, application of genetic modification techniques to GS cells with non-DBA/2 backgrounds extends the potential of a SSC-based approach in male germline modification.
- 2010-02-01
著者
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TAKASHIMA Seiji
Department of Cardiovascular Medicine, Osaka University Graduate School of Medicine
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Sado Takashi
Department Of Molecular Genetics Graduate School Of Medicine Kyoto University
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Miki H
The Institute For Physical And Chemical Research (riken) Bioresource Center
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Takashima Seiji
Department Of Cardiology Osaka University Graduate School Of Medicine
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Ogura Atsuo
The Institute For Physical And Chemical Research (riken) Bioresource Center
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Ogura Atsuo
Riken Bioresource Center
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Inoue Kimiko
The Institute For Physical And Chemical Research (riken) Bioresource Center
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Ogonuki Narumi
The Inst. For Physical And Chemical Res. (riken) Bioresource Center
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KANATSU SHINOHARA
Department of Molecular Genetics, Graduate School of Medicine, Kyoto University
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MIKI Hiromi
The Institute for Physical and Chemical Research (RIKEN), Bioresource Center
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MORIMOTO Hiroko
Department of Molecular Genetics, Graduate School of Medicine, Kyoto University
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SHINOHARA Takashi
Department of Molecular Genetics, Graduate School of Medicine, Kyoto University
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Ogonuki Narumi
Bioresource Center Riken
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Miki Hiromi
The Institute For Physical And Chemical Research (riken) Bioresource Center
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Morimoto Hiroko
Department Of Molecular Genetics Graduate School Of Medicine Kyoto University
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Morimoto Hiroko
Department Of Mathematics School Of Science And Technology Meiji University
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Kanatsu Shinohara
Department Of Molecular Genetics Graduate School Of Medicine Kyoto University
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Shinohara Takashi
Department Of Molecular Genetics Graduate School Of Medicine
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Kanatsu-Shinohara Mito
Department of Molecular Genetics, Graduate School of Medicine, Kyoto University
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