The Developmental Ability of Vitrified Oocytes from Different Mouse Strains Assessed by Parthenogenetic Activation and Intracytoplasmic Sperm Injection

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概要

• 論文の詳細を見る

• Assessment of the developmental ability of oocytes following freezing and thawing is an important step for optimizing oocyte cryopreservation techniques. However, the in vitro fertilization of frozen-thawed mouse oocytes is often inefficient because of incomplete capacitation of spermatozoa in the absence of surrounding cumulus cells. This study was undertaken to determine whether the oocyte cryopreservation efficiency of different strains of mice could be assessed from the development of oocytes following parthenogenetic activation and intracytoplasmic sperm injection (ICSI). Oocytes were collected from hybrid (C57BL/6 × DBA/2) F1 or inbred (C57BL/6J, C3H/HeN, DBA/2J and BALB/cA) strains and were vitrified in a solution containing ethylene glycol, DMSO, Ficoll and sucrose. In the first series of experiments, oocytes were activated parthenogenetically by Sr2+ treatment after warming. The oocytes of from the inbred strains, but not those of the F1 hybrid, were diploidized by cytochalasin treatment to obtain a sufficient number of blastocysts. In all strains tested, parthenogenetic embryos derived from vitrified oocytes developed into blastocysts at rates between 23% and 68%. In the second series of experiments, vitrified oocytes from each strain were injected with homologous spermatozoa after warming. Normal offspring were obtained from all strains at rates between 5% and 26% per embryo transferred. Thus, the feasibility of oocyte cryopreservation protocols can be assessed easily by in vitro development of parthenogenetic embryos or by in vivo development of ICSI embryos. Moreover, the oocytes of these four major inbred strains of mice can be cryopreserved safely for production of offspring.

• 日本繁殖生物学会の論文
• 2007-12-01

著者

• Ogura Atsuo

  The Institute For Physical And Chemical Research (riken) Bioresource Center

• Ogura Atsuo

  Riken Bioresource Center

• Mochida Keiji

  国立感染症研究所 免疫部

• Ogonuki Narumi

  The Inst. For Physical And Chemical Res. (riken) Bioresource Center

• KASHIWAZAKI Naomi

  Graduate School, Azabu University

• Ogonuki Narumi

  Bioresource Center Riken

• MOCHIDA Keiji

  RIKEN BioResource Center

• Kashiwazaki Naomi

  Graduate School Of Veterinary Medicine Azabu University

• ITO Masao

  Faculty of Bioindustry, Tokyo University of Agriculture

• ENDOH Keisuke

  RIKEN Bioresource Center

• OGONUKI Narumi

  RIKEN Bioresource Center

• OHKAWA Mika

  RIKEN Bioresource Center

• SHINMEN Akie

  RIKEN Bioresource Center

• Ito Masao

  Faculty Of Bioindustry Tokyo University Of Agriculture

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• The Developmental Ability of Vitrified Oocytes from Different Mouse Strains Assessed by Parthenogenetic Activation and Intracytoplasmic Sperm Injection
• Preface
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• Fertilization and preimplantation development of mouse oocytes after prolonged incubation with caffeine
• Optimization of a Protocol for Cryopreservation of Mouse Spermatozoa Using Cryotubes
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