Tracing Metabolic Changes in Soybean Cotyledons during Germination by NMR(Biological Chemistry)

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The changes in ^<13>C-NMR and ^<31>P-NMR spectra and ^1H-NMR images in soybean cotyledons during germination were investigated. Using ^<13>C-NMR, fatty acid signals in the form of triglycerides were observed in dry seeds, and those were observed approximately 18 days after the start of imbibition. Sucrose signals appeared at 16 hr and disappeared at 5 days. A -N^^+-(CH_3)_3 signal was observed after 5 days, suggesting the activation of membrane metabolism in the cotyledons. ^<31>P-NMR signals appeared 2 hr after imbibition before any apparent change in the ^<13>C-NMR spectrum. The peaks identified as sugar phosphate, inorganic phosphate in the cytoplasm and in the vacuole, and an unassigned compound, were distinguishable after 5 days. The vacuole-associated inorganic phosphate peak became prominent 18 days after imbibition in ^<31>P-NMR. Distribution maps of free water indicated that the stored macro-molecular materials which bound water were consumed heterogeneously within the cotyledon. The relaxation time (T_1) increased suddenly between 18 days and 23 days after imbibition, which indicates the consumption of stored materials. These findings suggest that cotyledons are the source of such compounds and the energy required for plant growth for approximately 18 days from germination until tri-foliolate leaves begin developing.
社団法人日本農芸化学会の論文
1990-06-23