Subsite Structure and Mode of Action of Native and Modified α-Amylase from Poricine Pancreas(Biological Chemistry)

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概要

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• The subsite structure of an active component of porcine pancreatic α-amylase, called PPA II, and modified PPA II (M_2) obtained with 5,5'-dithiobis-(2-nitrobenzoic acid) (DTNB), were setimated from their mode of action and rate parameters of hydrolyses of maltooligosaccharides. These results indicated that the native and modified PPA II have five subsites withe the catalytic site located between the third and fourth subsites from the nonreducing end. The subsite structure calculated for M_2 differed from that of PPA II; subsite affinities of PPA II were calculated to be 1.6, 3.9 and 2.8 kcal/mol for subsites 1, 2 and 5, respectively, whereas corresponding values for M_2 were 1.5, 1.4 and 2.6 kcal/mol, respectively. The sum of affinities of the third and fourth subsites was calculated to be -3.6 kcal/mol for PPA II and -2.1 kcal/mol for M_2, respectively, Evidently the mode of action of PPA II was changed by the introduction of bulky TNB^- ion at or near subsite 2. The difference of the mode of action between PPA II and M_2 was interpreted in terms of the structure of each subsite.

• 社団法人日本農芸化学会の論文
• 1986-12-23

著者

• Kobayashi Toshitaka

  Central Research Institute Meiji Milk Products Co. Ltd.

• Kobayashi T

  Nagoya Univ. Nagoya Jpn

• SAKANO Yoshiyuki

  Laboratory of Biological Chemistry, Department of Agricultural Chemistry, Faculty of Agriculture, To

• ISHIZUKA Yasuyuki

  Laboratory of Biological Chemistry, Department of Agricultural Chemistry, Faculty of Agriculture, To

• KOBAYASHI Tsuneo

  Laboratory of Biological Chemistry, Department of Agricultural Chemistry, Faculty of Agriculture, To

• Ishizuka Yasuyuki

  Laboratory Of Biological Chemistry Department Of Agricultural Chemistry Faculty Of Agriculture Tokyo

• Sakano Y

  Department Of Applied Biological Science Faculty Of Agriculture Tokyo University Of Agriculture And

• Sakano Yoshiyuki

  Laboratory Of Biochemistry Department Of Agricultural Chemistry Tokyo University Of Agriculture And

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