Elevated Post-Translational Modification of Proteins by O-Linked N-Acetylglucosamine in Various Tissues of Diabetic Goto-Kakizaki Rats Accompanied by Diabetic Complications
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概要
- 論文の詳細を見る
We aimed to examine the changes of O-GlcNAc modification of proteins in diabetes, especially in the pathogenesis of diabetic complications. The expression of O-GlcNAc-modified proteins and O-GlcNAc transferase was examined by Western blot analysis and immunohistochemically in various tissues (sciatic nerve, kidney, retina, liver, skeletal muscle, white adipose tissue, skin) of diabetic Goto-Kakizaki (GK) rats and nondiabetic Wistar rats. In the diabetic GK rats the Western blot bands of sciatic nerve, kidney and liver increased in the intensity. In all of the tissues of the nondiabetic Wistar rats, the O-GlcNAc and O-GlcNAc transferase immunoreactivities were observed in almost all cells, with strong expression indicated in the nucleus and weak expression in the cytoplasm. In the diabetic GK rats, these immunoreactivities in the sciatic nerve, kidney, and liver increased in intensity. In these tissues, morphological abnormalities were also observed at both light and electron microscopic levels. In contrast, the Western blot bands and immunoreactivities in the other tissues did not change in the diabetic GK rats. Furthermore, no morphological changes were observed in these tissues. These results indicate that elevated O-GlcNAc modification of proteins may be involved in the diabetic complications seen in diabetic GK rats.
- 日本組織細胞化学会の論文
著者
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Akimoto Yoshihiro
Department of Anatomy, Kyorin University School of Medicine
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Kawakami Hayato
Department of Anatomy, Kyorin University School of Medicine
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HIRANO Hiroshi
Department of Anatomy, Kyorin University School of Medicine
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Hart G
Department Of Biological Chemistry Johns Hopkins School Of Medicine
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Yamamoto Koji
Medical Informatics, Kochi Medical School
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Kawakami Hayato
Second Dep. Of Anatomy Dep. Of Biochemistry Kyorin Univ. School Of Medicine
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Kawakami Hayato
Dept. Of Anatomy Kyorin University School Of Medicine
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Kawakami Hayato
Department Of 2nd Anatomy Kyorin University School Of Medicine
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Akimoto Yoshihiro
Department Of Anatomy Kyorin University School Of Medicine
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Hirano Hiroshi
Food Research Lab. Toyama Food Research Institute
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Hirano Hiroshi
Second Department Of Pathology Hyogo College Of Medicine
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Hirano H
Department Of Anatomy Kyorin University School Of Medicine
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Kawakami H
Department Of Anatomy Kyorin University School Of Medicine
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Munetomo Eiji
Medicinal Research Laboratories, Taisho Pharmaceutical Co., Ltd.
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Wells Lance
Johns Hopkins School of Medicine, Department of Biological Chemistry
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Vosseller Keith
Johns Hopkins School of Medicine, Department of Biological Chemistry
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Hart Gerald
Johns Hopkins School of Medicine, Department of Biological Chemistry
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Wells Lance
Johns Hopkins School Of Medicine Department Of Biological Chemistry:(present Address)biochemistry &a
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Munetomo Eiji
Medicinal Research Laboratories Taisho Pharmaceutical Co. Ltd.
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Vosseller Keith
Johns Hopkins School Of Medicine Department Of Biological Chemistry:(present Address)department Of P
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Akimoto Y
Department Of Anatomy Kyorin University School Of Medicine
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Hirano H
Univ. Hyogo Hyogo
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Hirano Hiroshi
Department Of Anatomy Kansai Medical School
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Hirano Hiroshi
Department Of 2nd Anatomy Kyorin University School Of Medicine
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