Identification and Properties of the Glucose Transporter of Human Erythrocytes(MEMBRANE PROTEINS AND TRANSPORT-II)
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概要
- 論文の詳細を見る
Several lines of studies were undertaken to clarify the identity of the glucose transporter of human erythrocytes. Peptide maps of zone 4.5 which is the main component of the purified transporter fraction, were different from those of band 3. Cytochalasin B bound to the purified transporter fraction but not to band 3. Antibody raised against the purified transporter fraction cross-reacted with zone 4.5 and moderately with band 7, but not with other erythrocyte membrane proteins. These results indicate that zone 4.5 is the transporter (or a part of the transporter) and is not a fragment of band 3. With ferritin antibody electron microscopy and freeze-fracture electron microscopy, the glucose transporter were found to evenly distributed in reconstituted liposomes. Further morphological analysis coupled with transport assays showed the distribution of the transporters was random and was satisfactorily fitted to Poisson distribution, indicating reversible association of the transporters does not occur in liposomes and is not necessary for transport activity. This communication summarises our recent studies on identification and properties of the glucose transporter of human erythrocytes. A full account of the studies is published elsewhere (22, 23).
- 東海大学の論文
- 1982-06-14
著者
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OSUMI MASAKO
Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University
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HIRANO Hiroshi
Department of Anatomy, Kyorin University School of Medicine
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Takata Kuniaki
Department of Anatomy and Cell Biology, Gunma University School of Medicine
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Osumi Masako
Department Of Chemical And Biological Sciences Faculty Of Science Japan Woman's University
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Takata Kuniaki
Department Of Anatomy And Cell Biology Gunma University Graduate School Of Medicine
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KASAHARA Michihiro
Department of Biology, Faculty of Science, University of Tokyo
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NAGANO Misuzu
Department of Biology, Japan Women's University
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Kasahara Michihiro
Department Of Biology Faculty Of Science University Of Tokyo
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Nagano Misuzu
Department Of Biology Japan Women's University
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Osumi Masako
Department Of Biology Faculty Of Science Osaka City University
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Hirano Hiroshi
Department Of Anatomy Kansai Medical School
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Sase Sin'ichi
Department Of Biology Faculty Of Science University Of Tokyo
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Hirano Hiroshi
Department Of 2nd Anatomy Kyorin University School Of Medicine
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Hirano Hiroshi
杏林大学 解剖
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