Biochemical and Electron-Microscopic Evidence for Membrane Injury in Yeast Cells Quickly Frozen with Liquid Nitrogen

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概要

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• We obtained basic information on verifying the mechanisms of injury caused by intracellular ice formation from fast cooling with liquid nitrogen (LN_2) in yeast. Suspensions of the liquid nitrogen-treated yeast cultures containing [^3H]-labeled deoxy-ribonucleic acid were incubated with added DNase in a buffer solution. Extracellular radioactivity levels increased in solutions of the LN_2-treated cells, although the activities for control cells treated at 0℃ were almost unchanged. We considered that the membrane systems of the LN_2-treated cells were damaged and the permeability barrier destroyed, but that this was not the case in 0℃-treated cells. Furthermore we suggested that native DNase worked effectively on its DNA after the LN_2-treatment. These considerations were confirmed by electron microscopy. Thus, While the ultra structure of 0℃-treated cells was not changed, the cell membrane and nuclear membrane of the LN_2-treated cells were seriously damaged.

• 社団法人日本生物工学会の論文
• 1987-04-25

著者

• OSUMI MASAKO

  Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University

• Sato M

  Department Of Biology Japan Women's University

• Sato Mamiko

  Department Of Chemical And Biological Sciences Faculty Of Science Japan Women's University

• KOMATSU YASUHIKO

  Fermentation Research Institute Agency of Industrial Sience and Technology, Ministry of Internationa

• KOMATSU Yasuhiko

  Pharmaceuticals and Biotechnology Laboratory, Japan Energy Corporation

• Komatsu Y

  National Inst. Advanced Ind. Sci. And Technol. Ibaraki Jpn

• Osumi Masako

  Department Of Biology Japan Women's University

• Osumi Masako

  Department Of Biology Faculty Of Science Osaka City University

• OSUMI Masako

  Department of Biology, Japan Women's University

• SATO MAMIKO

  Department of Biology, Japan Women's University

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