Responsiveness of yeast cells to auxin in relation to cell age
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概要
- 論文の詳細を見る
Using Urografin density gradient centrifugation, we fractionated cells in a stationary culture of a homothallic strain, D6-0, of Saccharomyces cerevisiae and obtained two groups; a large cell fraction (L fraction) and a small cell fraction (S fraction). Indole-3-acetic acid (20 mg/liter) expands cells of the S fraction but not those of the L fraction. Differences in the physiological nature between the two fractions were studied in relation to their responsiveness to auxin. In general, L cells contain larger amounts of DNA, RNA and protein per cell than do S cells. When cells of the two fractions were transferred to a nutrient medium, S fraction showed a longer lag period than L fraction before buds appeared on cells. Preceding cell division, cells of S fraction enlarged significantly, while cells of L fraction enlarged only slightly. In the 4 hr following inoculation, DNA content per culture doubled in the L fraction while the increase was very small in the S fraction. Electron microscopy revealed that cell walls were thicker in the S fraction than in the L fraction. Development of mitochondria and other intracellular membraneous structures was poorer in cells of the S fraction than in those of the L fraction. The relationship between the cell generation cycle and responsiveness to auxin is discussed.
- 日本植物生理学会の論文
著者
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OSUMI MASAKO
Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University
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SHIMODA CHIKASHI
Department of Biology, Graduate School of Science, Osaka City University
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Yanagishima Naohiko
Department of Biology, Nagoya University
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Osumi Masako
Department Of Chemical And Biological Sciences Faculty Of Science Japan Woman's University
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Yanagishima Naohiko
Department Of Biology Faculty Of Science Osaka City University
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Yanagishima Naohiko
Department Of Biology Japan Women's University
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Shimoda Chikashi
Department Of Biology Faculty Of Science Osaka City University
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Osumi Masako
Department Of Biology Faculty Of Science Osaka City University
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