Nucleation of Astral-shaped Microtubules from Latex Beads Conjugated with MTOG Proteins(Cell Biology)
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概要
- 論文の詳細を見る
A model system for the formation of astral-shaped microtubules (Mts) consisting of Latex beads (diameter of 0.2 μm), a protein fraction (p51) comprised of MTOGs (microtubule-organizing granules) and tubulin was established. The Latex beads were first incubated with p51 in the presence of GTP at 0℃, then the purified tubulin dimer fraction was added, resulting in the formation of an aster-like structure observed by dark-field microscopy. On preincubation of the Latex beads with GDP instead of GTP, the asters did not form. Unhydrolyzable GTP analogues such as GTP-γS and GMP-PNP promoted aster formation as did GTP as observed by dark-field microscopy. Polylysine, as representative of basic polymers capable of binding to the surface of the Latex beads, promoted spontaneous Mt assembly, and eventually an aster-like structure without Latex beads in the center formed. Further analyses made by measuring the optical density of the aster-forming system produced the following results. 1) preincubation of the Latex beads with GTP or GMP-PNP supported Mt assembly from the beads showing profiles typical for a sitedirected assembly without the lag phase. 2) GTP-γS and GDP inhibited the turbidity increase of the system, causing a decrease in both the initial velocity and the level of steady state of Mt assembly. 3) Anti-p51 monoclonal antibody (HP1) substantially inhibited the aster formation, while anti-γ-tubulin antibody only slightly inhibited assembly.
- 社団法人日本動物学会の論文
- 2000-07-01
著者
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OSUMI MASAKO
Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University
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TAKAHASHI Kyoko
Department of Food Science and Nutrition, School of Human Environmental Sciences, Mukogawa Women’s U
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Nakamura Akiko
Department of Surgery, Kagoshima Medical Association Hospital
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OSUMI Masako
日本女子大学理学部
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Osumi Masako
Department Of Chemical And Biological Sciences Faculty Of Science Japan Women's University
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Osumi Masako
Laboratory Of Electron Microscopy/open Research Center Japan Women's University
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Osumi Masako
Department Of Chemical And Biological Sciences Faculty Of Science Japan Woman's University
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大隈 正子
日本女子大学理学部物質生物科学科
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大隅 正子
Department Of Chemical And Biological Sciences Faculty Of Science Japan Women's University
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Sato Mamiko
Department Of Chemical And Biological Sciences Faculty Of Science Japan Women's University
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Sakai H
Department Of Applied Aquabiology National Fisheries University
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SAKAI Hikoichi
Department of Chemical and Biolobical Sciences, Faculty of Science, Japan Women's University
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Maruno Ayako
Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University
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Sugawara Natsuki
Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University
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Hoshi Chihiro
Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University
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Nakamura Risako
Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University
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Shinozaki Naeko
Department of Chemical and Biological Sciences, Faculty of Science, Japan Women's University
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Maruno Ayako
Department Of Chemical And Biological Sciences Faculty Of Science Japan Women's University
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Sugawara Natsuki
Department Of Chemical And Biological Sciences Faculty Of Science Japan Women's University
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Nakamura Risako
Department Of Chemical And Biological Sciences Faculty Of Science Japan Women's University
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Takahashi Kyoko
Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'
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Osumi Masako
Department Of Biology Faculty Of Science Osaka City University
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Takahashi Kyoko
Department Of Chemical And Biological Sciences Faculty Of Science Japan Women's University
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Hoshi Chihiro
Department Of Chemical And Biological Sciences Faculty Of Science Japan Women's University
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Shinozaki Naeko
Department Of Chemical And Biological Sciences Faculty Of Science Japan Women's University
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Nakamura Akiko
Department Of Chemical And Biological Sciences Faculty Of Science Japan Women's University
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Nakamura Akiko
Department Of Bioscience And Technology Faculty Of Agriculture Iwate University
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Sakai Hikoichi
Department Of Biophysics And Biochemistry Faculty Of Science The University Of Tokyo
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Takahashi Kyoko
Department Of Bioproduction Science Faculty Of Horticulture Chiba University
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NAKAMURA Akiko
Department of Bacteriology, National Institute of Health
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SATO MAMIKO
Department of Biology, Japan Women's University
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