Molecular Cloning of a cDNA that Encodes a Spinach Cysteine Synthase-Like Protein

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概要

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• In Spinacia oleacea, three isoforms of cysteine synthase (CSase, O-acetyl-serine (thiol) lyase) were identified in the cytosolic, plastidial, and mitochondorial compartments. Here, we report molecular cloning of a cDNA that encodes a CSase-like protein, designated CSaseLP. The predicted amino acid sequence of CSaseLP showed high identity (about 70%) with that of a spinach cytosolic CSase (encoded by CSaseA gene). The CSase activity of recombinant CSaseLP was barely detectable. The antibody raised against the recombinant CSaseLP protein recognized weakly a recombinant CSaseA protein. Northern blot analysis showed that CSaseLP gene was expressed mainly in root tissues, while the expression of CSaseA gene was constitutive in leaf and root tissues. These results suggest that the CSaseLP protein, though belongs to the CSase family, play a role distinct from the cytosolic CSase.

• 日本植物細胞分子生物学会の論文
• 2004-03-01

著者

• KODAMA Hiroaki

  Department of Chemistry, Faculty of Science and Engineering, Saga University

• ASAI Ryosuke

  Department of Biochemistry and Department of Molecular Biology, Nippon Medical School

• TAKAHASHI Kyoko

  Department of Food Science and Nutrition, School of Human Environmental Sciences, Mukogawa Women’s U

• Asai Ryosuke

  Department Of Biochemistry And Department Of Molecular Biology Nippon Medical School

• Asai Ryosuke

  Department Of Bioproduction Science Faculty Of Horticulture Chiba University

• MASADA Masahiro

  Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University

• NAKATA Junko

  Department of Neurobiology, Kyoto Pharmaceutical University

• Kodama Hiroaki

  Department Of Bioproduction Science Faculty Of Horticulture Chiba University

• Kodama Hiroaki

  Department Of Anatomy Ohu University School Of Dentistry

• Nakata Junko

  Department Of Bioproduction Science Faculty Of Horticulture Chiba University

• Takahashi Kyoko

  Department Of Food Science And Nutrition School Of Human Environmental Sciences Mukogawa Women'

• Masada Masahiro

  Department Of Biology Tokyo Metropolitan University

• Masada Masahiro

  Department Of Bioproduction Science Faculty Of Horticulture Chiba University

• Takahashi K

  Department Of Bioproduction Science Faculty Of Horticulture Chiba University

• Takahashi Kyoko

  Department Of Chemical And Biological Sciences Faculty Of Science Japan Women's University

• Takahashi Kyoko

  Department Of Bioproduction Science Faculty Of Horticulture Chiba University

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