Measurement of Serine Acetyltransferase Activity in Crude Plant Extracts by a Coupled Assay System Using Cysteine Synthase

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概要

• 論文の詳細を見る

• Serine acetyltransferase (SATase) (EC 2.3.1.30) catalyzes the formation of O-acetyl-_L-serine (OAS) from _L-serine in the presence of acetyl-CoA. A novel assay method was developed for measuring this enzyme activity in extracts from plant tissues. The assay consists of a coupled system in which the OAS formed is converted to cysteine by the addition of cysteine synthase (CSase) (EC 4.2.99.8). Cysteine thus formed is determined colorimetrically and serves as a measure for SATase activity. This method is rapid, simple and sensitive, and can be readily adapted for measurement of SATase activity in crude tissue extracts or homogenates.

• 日本植物生理学会の論文

著者

• Nakamura K

  Nagoya Univ. Nagoya Jpn

• Fukushima K

  Nihon Univ. School Of Dentistry At Matsudo Matsudo Jpn

• MASADA Masahiro

  Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University

• Fukushima Kazuo

  Department of Microbiology, Nihon University School of Dentistry at Matsudo

• TAMURA Goro

  Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University

• Tamura Goro

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• Tamura Goro

  Department Of Agricultural Chemistry Faclty Of Horticulture Chiba University

• Nakamura K

  Lab. Biochem. Grad. School Of Bioagr. Sci. Nagoya Univ.:dept. Developmental Biol. Natl. Inst. Basic

• Nakamura Katsuhito

  Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University

• Hayama Atsushi

  Department of Agricultural Chemistry, Faculty of Horticulture, Chiba University

• Hayama Atsushi

  Department Of Agricultural Chemistry Faculty Of Horticulture Chiba University

• Nakamura Katsuhito

  Department Of Agricultural Chemistry Faculty Of Horticulture Chiba University

• Fukushima Kazuo

  Department Of Environmental Sciences Faculty Of Science Shinshu University

• Nakamura Kenzo

  Department Of Biological Mechanisms And Functions Graduate School Of Bioagricultural Sciences Nagoya

• Tamura G

  Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University

• Masada M

  Chiba Univ. Chiba Jpn

• Masada Masahiro

  Department Of Biology Tokyo Metropolitan University

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