Purification and Characterization of Novel Cysteine Synthase Isozyme from Spinach Hydrated Seeds
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概要
- 論文の詳細を見る
A novel type of cysteine synthase (CSase, Ec 4.2.99.8) isozyme, designated as CSase 1', was purified to homogeneity from hydrated spinach seeds.The enzyme had a molecular weight of 68, 000 and consisted of two identical subunits of Mr 34, 000.The apparentK_m for O-acetyl-L-serine was 8.33 mM and that for sulfide was 0.66 mM.The activity of CSase 1' was maintained when it was treated at 60°C for 1 min.This novel enxyme was similar to CSases 1, 2, and 3 already purified from spinach leaves, in results of double immunodiffusion, molecular weight, subunit composition, K_m values for O-acetyl-L-serine and sulfide, and heat stability.One the other hand, N-terminal amino acid sequence, effects of immunotitration, pH optimum, and effects of hydroxylamine on purified CSase 1' were different from those of the other CSase, Furthermore, it was found that CSases 2S and 3S isolated from hydrated spinach seeds were identical with the CSases 2 and 3 reported previlously.It was also disclosed that CSases 1, 2, and 3 were localized in chloroplasts, cytosol, and mitochondria, respectively.
- 社団法人日本農芸化学会の論文
- 1998-03-23
著者
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YAMAGUCHI Takayuki
Department of Physics, Saitama University
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Zhu Xia
Graduate School Of Science And Engineering Ehime University
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Zhu Xia
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University
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Yamaguchi T
Chiba Univ. Chiba Jpn
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MASADA Masahiro
Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University
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Yamaguchi Takayuki
Department Of Bioresources Chemistry Faculty Of Horticulture Chiba University Engineering Department
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Yamaguchi Taichi
Laboratory Of Biophysical Chemistry College Of Agriculture Osaka Prefecture University
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Yamaguchi T
Department Biochemistry National Institute Of Agrobiological Science
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Masada M
Chiba Univ. Chiba Jpn
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Masada Masahiro
Department Of Biology Tokyo Metropolitan University
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Zhou Xia
Department of Bioresources Chemistry, Faculty of Horticulture, Chiba University
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