Purification and Characterization of an Oligo-isomaltosaccharide Synthase from a Streptococcus sobrinus Glucosyltransferase-I Deficient Mutant

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概要

• 論文の詳細を見る

• One glucosyltransferase (GTF)-I deficient mutant of Streptococcus sobrinus strain B13N was isolated through chemical mutagenesis with ethyl methanesulfonate, and characterized. This mutant, designated as B13N-Id, readily allowed us to purify a homogeneous oligo-isomaltosaccharide synthase (GTF-S) from its culture fluid. The purified GTF-S was only recognized with rabbit polyclonal antibody against recombinant GUT-S from an Ecsherichia coli MD124 clone expressing the B13N gtfS gene, and showed the almost same enzymatic properties as the recombinant enzyme. A double reciprocal plot of the B13N GTF-S for sucrose was biphasic, and the affinity for this substrate was high compared to that of GTF-S enzymes from other strains.

• 社団法人日本農芸化学会の論文
• 2001-06-23

著者

• ABIKO Yoshimitsu

  Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo

• Fukushima K

  Nihon Univ. School Of Dentistry At Matsudo Matsudo Jpn

• Fukushima Kazuo

  Department of Microbiology, Nihon University School of Dentistry at Matsudo

• Hayakawa Manami

  Department Of Agricultural Chemistry Kyushu University

• Abiko Yoshimitsu

  Department Of Biochemistry And Molecular Biology School Of Dentistry At Matsudo Nihon University

• SHIROZA Teruaki

  Department of Chemistry, Nihon University School of Dentistry at Matsudo

• HAYAKAWA Mitsuo

  Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo

• SHINOZAKI-KUWAHARA Noriko

  Departments of Microbiology, Nihon University school of Dentistry at Matsudo

• Shinozaki Noriko

  Department Of Microbiology Nihon University School Of Dentistry At Matsudo Matsudo

• Shinozaki-kuwahara Noriko

  Departments Of Microbiology Nihon University School Of Dentistry At Matsudo

• Abiko Yoshimitsu

  Department Of Biochemistry And Molecular Biochemistry Nihon University School Of Dentistry At Matsud

• Fukushima Kazuo

  Department Of Environmental Sciences Faculty Of Science Shinshu University

• Shiroza Teruaki

  Department Of Chemistry Nihon University School Of Dentistry At Matsudo

• Shinozaki-Kuwahara Noriko

  Department of Microbiology, Nihon University School of Dentistry at Matsudo, Matsudo

• Shinozaki-Kuwahara Noriko

  Department of Microbiology and Immunology, Nihon University School of Dentistry at Matsudo

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