Gene Expression of MC3T3-E1 Cells on Fibronectin-immobilized Titanium Using Tresyl Chloride Activation Technique
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概要
- 論文の詳細を見る
Fibronectin (FN) can be immobilized directly on titanium surfaces using tresyl chloride activation technique. The key advantage of tresyl chloride activation technique lies in its simplicity. In this study, we examined the cell attachment and gene expression of MC3T3-E1 cells on FN-immobilized titanium using GeneChip. Cells attached on FN-immobilized titanium at a higher rate than untreated titanium. FN altered the gene expression profile, whereby 62 genes were found to be up-regulated, while 56 genes were found to down-regulate to over twice the level on day 14. FN not only enhanced the expression levels of IBSP and OMD, but also decreased SULF1 mRNA level. Taken together, the immobilization of FN on tresylated titanium promoted early matrix mineralization and bone formation.
- 日本歯科理工学会の論文
著者
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YOSHINARI Masao
Department of Dental Materials Science, Tokyo Dental College
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TSUTSUMI Haruhiko
Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo
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PUGDEE Kamolparn
Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo
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HAYAKAWA Tohru
Department of Dental Biomaterial, Nihon University School of Dentistry at Matsudo
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ABIKO Yoshimitsu
Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo
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SHIBATA Yasuko
Department of Biochemistry and Molecular Biology, School of Dentistry at Matsudo, Nihon University
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Shibata Yasuko
日本大学 薬学部臨床医学ユニット
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Tsutsumi Haruhiko
Department Of Biochemistry And Molecular Biology Nihon University School Of Dentistry At Matsudo
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Hayakawa Tohru
Department Of Dental Biomaterial Nihon University School Of Dentistry At Matsudo
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Hayakawa Tohru
Nihon Univ. Graduate School Of Dentistry At Matsudo Chiba Jpn
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Hayakawa Tohru
Department Of Appilied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Shibata Yasuko
Department Of Biochemistry And Molecular Biology Nihon University School Of Dentistry At Matsudo:res
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Yoshinari Masao
Department Of Dental Materials Science And Oral Health Science Center Tokyo Dental College
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Yoshinari Masao
Division Of Oral Implants Research Oral Health Science Center Tokyo Dental College
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Pugdee Kamolparn
Department Of Biochemistry And Molecular Biology Nihon University School Of Dentistry At Matsudo
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Abiko Yoshimitsu
Department Of Biochemistry Nihon University School Dentistry At Matsudo
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Abiko Y
Department Of Biochemistry And Molecular Biology Nihon University School Of Dentistry At Matsudo:res
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Abiko Yoshimitsu
Department Of Biochemistry And Molecular Biochemistry Nihon University School Of Dentistry At Matsud
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YAMAMICHI Nobuyuki
Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo
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Yamamichi Nobuyuki
Department Of Biochemistry And Molecular Biology Nihon University School Of Dentistry At Matsudo
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