Polycationic protamine for water-insoluble complex formation with DNA
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概要
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The DNA/protamine complex was prepared by a reaction between DNA and protamine sulfate solutions with stirring, and its cell viability, antibacterial effect and histopathological responses were examined. A water-insoluble white powder, DNA/protamine complex, with a porous structure was obtained. The molar binding ratio of the complex prepared from a solution containing equal amounts of DNA and protamine sulfate by weight was 0.038 and the efficiency of complex formation was 61%. In a cell culture test using MC-3T3-E1 mouse osteoblast cells, the complex showed less cytotoxicity than protamine sulfate alone and cell viabilities were more than 98%. A porous disk could be prepared easily and showed an antibacterial effect against Staphyrococcus aureus, Porphyromonas gingivalis and Prevotella intermedia in an antibacterial sensitivity test and a mild tissue response in vivo test. These results suggested that the DNA/protamine complex could be a useful biodegradable biomaterial with antibacterial effects.
- 2010-09-01
著者
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HAYAKAWA Tohru
Department of Dental Biomaterial, Nihon University School of Dentistry at Matsudo
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DOI Yutaka
Department of Dental Materials Science, Division of Oral Functional Science and Rehabilitation, Asah
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FUKUSHIMA Tadao
Department of Dental Engineering, Bioengineering section, Fukuoka Dental College
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OHNO Jun
Department of Morphological Biology, Pathology section, Fukuoka Dental College
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IMAYOSHI Rieko
Department of Functional Bioscience, Infection Biology Section, Fukuoka Dental College
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KAWAGUCHI Minoru
Department of Dental Engineering, Bioengineering section, Fukuoka Dental College
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KANAYA Keiichi
Department of Periodontology, Division of Oral Infections and Health Sciences, Asahi University Scho
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MITARAI Makoto
Central Research Institute, Maruha-Nichiro Holdings Inc.
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Hayakawa Tohru
日本大学松戸歯学部 歯科生体材料学
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Hayakawa Tohru
Department Of Appilied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Fukushima Tadao
Department Of Dental Engineering Bioengineering Section Fukuoka Dental College
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Fukushima Tadao
Associate Professor Department Of Dental Engineering Biomaterials Section Fukuoka Dental College
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Ohno Jun
Department Of Morphological Biology Pathology Section Fukuoka Dental College
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Doi Yutaka
Department Of Dental Material And Technology Asahi University School Of Dentistry
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Mitarai Makoto
Central Research Institute Maruha-nichiro Holdings Inc.
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Imayoshi Rieko
Department Of Functional Bioscience Infection Biology Section Fukuoka Dental College
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Kanaya Keiichi
Department Of Periodontology Division Of Oral Infections And Health Sciences Asahi University School
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Fukushima Tadao
Department Of Dental Engineering Fukuoka Dental College
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Kawaguchi Minoru
Department Of Dental Engineering Bioengineering Section Fukuoka Dental College
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Doi Yutaka
Department Of Cardiology
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