The Influence of Cryopreservation on the Oligosaccharide Expression of Tooth Germ : Sugar Chain Expression of Frozen Tooth Germs(International Symposium of Maxillofacial and Oral Regenerative Biology in Okayama 2005)

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To explore the influence of preservation by freezing on tissues of tooth germ, we examined changes in histological architectures and glycoconjugate expression detected by lectins, in tooth germs, with or without freezing, of postnatal 5 day rats. The results acquired were as follows: tissue architectures and cellular morphologies were similar between frozen and control groups. Stellate cells of enamel medulla and pulpal cells were, however, slightly atrophic at the control group. With lectin staining, there are no remarkable changes between frozen and control groups. From these results, we conclude that the influence of preservation by freezing was relatively small.
硬組織再生生物学会の論文