Monitoring of DNA Complex Formation by Means of Quartz Crystal Microbalance
スポンサーリンク
概要
- 論文の詳細を見る
The double-helical structure of double-stranded DNA enables it to form complexes with antibiotics and cytokines. The present study investigated the complex formation for antibiotic or cytokine towards DNA using quarts-crystal microbalance (QCM) technique. The resonance frequency decreases in QCM system linearly upon the increase of adsorbed mass on the QCM sensor at the nanogram level. Biotinylated DNA can easily bind avidin molecules, and DNA could be immobilized onto QCM sensor, which was sputtered with gold. The crystal resonant frequency shift was immediately measured after the injection of daunorubicin hydrochloride, which was an antibiotics, and of basic fibroblast growth factor (b-FGF), which was a growth factor. The present study can monitor the complex formation between daunorubicin hydrochloride or b-FGF and DNA using QCM method, and binding amount of daunorubicin hydrochloride or b-FGF towards DNA can be easily estimated by the frequency decrease. In addition, the difference of binding mode of daunorubicin hydrochloride and b-FGF towards DNA was revealed by the frequency shift. The QCM method is a useful for monitoring DNA complex formation with antibiotics or cytokines.
- 日本再生歯科医学会の論文
- 2006-12-30
著者
-
HAYAKAWA Tohru
Department of Dental Biomaterial, Nihon University School of Dentistry at Matsudo
-
Taniguchi Kunihisa
Department Of Morphological Biology Pathology Section Fukuoka Dental College
-
Inoue Yusuke
Fukuoka College Of Health Sciences
-
FUKUSHIMA Tadao
Department of Dental Engineering, Bioengineering section, Fukuoka Dental College
-
KAWAGUCHI Minoru
Department of Dental Engineering, Bioengineering section, Fukuoka Dental College
-
Hayakawa Tohru
日本大学松戸歯学部 歯科生体材料学
-
KAMINISHI Hidenori
Department of Functional Bioscience, Section of Infection Biology, Fukuoka Dental College
-
Hayakawa Tohru
Department Of Appilied Biology Faculty Of Textile Science Kyoto Institute Of Technology
-
Fukushima Tadao
Department Of Dental Engineering Bioengineering Section Fukuoka Dental College
-
Fukushima Tadao
Associate Professor Department Of Dental Engineering Biomaterials Section Fukuoka Dental College
-
Fukushima Tadao
Department Of Dental Engineering Fukuoka Dental College
-
Kawaguchi Minoru
Department Of Dental Engineering Bioengineering Section Fukuoka Dental College
-
Taniguchi Kunihisa
Section Of Pathology Department Of Morphological Biology Division Of Pathology Department Of Morphol
-
Taniguchi Kunihisa
Department Of Morphological Biology Fukuoka Dental College
-
Kaminishi Hidenori
Department Of Functional Bioscience Section Of Infection Biology Fukuoka Dental College
-
Taniguchi Kunihisa
Department Of Biological Morphology Fukuoka Dental College
関連論文
- Discoidin Domain Receptor-1 Gene Expression of Osteoblasts on Fibronectin Coated Titanium Disks
- Thin Carbonate Apatite Layer Deposited on Titanium using Molecular Precursor Method
- Molecular Precursor Method Can Coat Thin Carbonate-containing Apatite (CA) Film inside Titanium Fiber Mesh
- Cell-adhesive Protein Immobilization Using Tresyl Chloride-activation Technique for the Enhancement of Initial Cell Attachment
- Frontier Molecular Orbital Study for the Reaction of Tresyl Chloride
- Deposition of Thin Calcium Phosphate Film onto Titanium Using RF Magnetron Sputtering Technique
- Localization of a novel 252-kDa plasma membrane protein that binds Cry1A toxins in the midgut epithelia of Bombyx mori
- Immobilization of Fibronectin onto Organic Hexamethyldisiloxane Coatings with Plasma Surface Modification : Analysis of Fibronectin Adsorption Using Quartz Crystal Microbalance-dissipation Technique
- X-ray Phase-Contrast Imaging and X-ray Absorption Fine Structure Analysis using Parametric X-ray Radiation Generated at LEBRA, Nihon University
- Polycationic protamine for water-insoluble complex formation with DNA
- A Clinical and Histopathological Study of Guided Bone Regeneration Using Polytetrafluoroethylene Membrane in the Wound Resulting from Human Tooth Extraction
- Non-linear Finite Element Analysis of the Failure Progression of Fiber-reinforced Ceramics Produced by Tape Casting Technique
- Bonding Durability between Orthodontic Brackets and Human Enamel treated with Megabond Self-etching Primer Using 4-META/MMA-TBB Resin Cement
- SEM Observation of Human Enamel and Resin Tag Formation After Phosphoric Acid Etching : Influence of Saliva Contamination
- Development of Self-etching Primer Adhesive in All-in-one Bonding System
- Tensile Bond Strength of 4-META/MMA-TBB Resin to Ground Bovine Enamel Using a Self-etching Primer
- Influence of Apatite Crystallinity in Porous PLGA/Apatite Composite Scaffold on Cortical Bone Response
- Pre-intercalated DNA/Lipid/PLGA Film as a Drug Carrier
- Complexation of DNA with Cationic Polyamino Acid for Biomaterial Purposes
- Preparation and Tissue Response of Lipid-coated b-FGF
- Drug Binding and Releasing Characteristics of DNA/Lipid/PLGA Film
- Monitoring of DNA Complex Formation by Means of Quartz Crystal Microbalance
- Preparation of Carbon Nanotube-alginate Nanocomposite Gel for Tissue Engineering
- PBS Buffer Solutions with Different pH Values Can Change Porosity of DNA-chitosan Complexes
- Antibacterial Activities and Bonding of MMA/TBB Resin Containing Amphiphilic Lipids
- A Novel 96-kDa Aminopeptidase Localized on Epithelial Cell Membranes of Bombyx mori Midgut, Which Binds to Cry1Ac Toxin of Bacillus thuringiensis
- Increase in Indole -3- acetic Acid (IAA) level and Nitrilase Activity in Turnips Induced by Plasmodiophora brassicae Infection
- Syntheses and Polymerizations of (Meth)acrylates Having Containing a Pendant Spiro Ortho Ester and Carbonate Moieties
- Mode Superposition Transient Dynamic Analysis for Dental Implants with Stress-absorbing Elements : A Finite Element Analysis
- Conformational and Quantum Analysis of Dental Adhesive Carboxylic Acid and Carboxylic Acid Anhydride Monomers
- Effectiveness of the Addition of Water-soluble Photoinitiator into the Self-etching Primers on the Adhesion of a Resin Composite to Polished Dentin and Enamel
- In vitro Study of Collagen Coating of Titanium Implants for Initial Cell Attachment
- Influence of Bovine Dentin Site on the Bond Strength of Resin Cement
- Application of High Resolution Microfocus X-ray CT for the Observation of Human Tooth
- The Influence of Cryopreservation on the Oligosaccharide Expression of Tooth Germ : Sugar Chain Expression of Frozen Tooth Germs(International Symposium of Maxillofacial and Oral Regenerative Biology in Okayama 2005)
- CO_2 Laser Irradiation of Palatal Bone in Rats(13^ Annual Meeting of the Society for Hard Tissue Regenerative Biology)
- A-58. Systematic Pituitary Function Tests of the Sellar and Parasellar Tumor Cases
- Enhancement of baculovirus infection in Spodoptera exigua (Lepidoptera: Noctuidae) larvae with Autographa californica nucleopolyhedrovirus or Nicotiana tabacum engineered with a granulovirus enhancin gene
- Comparison of Otoraplin Gene Expressions in Osteoblasts Grown on Fibronectin and GRGDSP Peptide-Coated Titanium Disks
- Physical Mapping of Bombyx mori Nuclear Polyhedrosis Virus Strain D1 : Identification of Novel Interspersed Homologous Regions
- Efficacy of Water-soluble Photoinitiator on the Adhesion of Composite Resin to Bovine Teeth in All-in-one Bonding System
- Gene expression monitoring in osteoblasts on titanium coated with fibronectin-derived peptide
- Effect of Oral Administration of High Advanced-Collagen Tripeptide (HACP) on Bone Healing Process in Rat
- A case of lymphoepithelioma-like carcinoma of the colon with ulcerative colitis
- Modification of 4-META/MMA-TBB Resin for Safe Debonding of Orthodontic Brackets : Influence of the Addition of Degradable Additives or Fluoride Compound
- Effect of Thermal Cycling on Shear Bond Strength with Different Types of Self-etching Primer for Bonding Orthodontic Brackets using a MMA-based Resin
- Influence of silane coupling agent on the bonding of orthodontic brackets to porcelain teeth
- Atomic Force Mcroscopic Images of Acidic Conditioning Agents on Various Regions of Human Dentin
- Physical map of a Plutella exlostella granulovirus genome
- Gene Expression of MC3T3-E1 Cells on Fibronectin-immobilized Titanium Using Tresyl Chloride Activation Technique
- Influence of the Human Skin Model on Cell Viability Test and the Formation Test of Tubule-like Structures by Very Low Concentrations Copper, Titanium and Zinc Ions
- Apatite Deposition on Several Dental Biodegradable Materials in Simulated Body Fluid
- Evaluation of resin infiltration at hybrid layer using Br-tracing method
- Study on Rat Subcutaneous Reaction to Experimental Polyurethane Elastomers
- Degradation of Methacrylate Monomers in Human Saliva
- Non-Hodgkin's Lymphoma of B-cell Lineage associated with Peculiar Eosinophilia : A Case Report
- 2. The relation between the timing of tooth germ injury and the disturbance of hard tissue formation of teeth.
- SEM analysis of microstructure of adhesive interface between resin cement and dentin treated with self-etching primer
- Fibronectin Adsorption onto Plasma-polymerized Hexamethyldisiloxnane Coating
- Conformational Analysis of Bisphosphonate/Calcium Complex
- Collagen Nanofiber on Titanium or Partially Stabilized Zirconia by Electrospray Deposition
- Influence of thickness and undercut of thermoplastic resin clasps on retentive force
- Adsorption study of pellicle proteins to gold, silica and titanium by quartz crystal microbalance method
- Efficacy of Self-etching Primer containing Water-soluble Monomers in the Adhesion of 4-META/MMA-TBB Resin to Bovine Ground Enamel