Immobilization of Fibronectin onto Organic Hexamethyldisiloxane Coatings with Plasma Surface Modification : Analysis of Fibronectin Adsorption Using Quartz Crystal Microbalance-dissipation Technique

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The immobilization behavior of fibronectin onto plasma-surface modified titanium was evaluated by using the quartz crystal microbalance-dissipation (QCM-D) technique. The plasma-surface modifications included a thin-film coating of hexame-thyldisiloxane (HMDSO) and an O_2-plasma treatment on titanium. The contact angle of HMDSO-coatings against double-distilled water was 100 degrees, and dramatically decreased after the O_2-plasma treatment with less than 5 degrees. XPS analysis indicated the introduction of an O_2-functional group onto the HMDSO surface by the O_2-plasma treatment. The QCM-D technique enabled evaluation the adsorption behavior of fibronectin. The largest amount of fibronectin adsorbed was on both as-coated HMDSO (hydrophobic surfaces) and O_2-plasma treated at 10-min (hydrophilic surfaces) surfaces compared to O_2-plasma treated (24-h) surfaces. These results indicated that two adsorption mechanisms of the fibronectin to substrates were considered, including hydrophobic interaction and ionic bond via OH group and/or O_2-functional group concomitant with hydrophilic surfaces.
日本再生歯科医学会の論文
2004-03-30