Gene expression monitoring in osteoblasts on titanium coated with fibronectin-derived peptide
スポンサーリンク
概要
- 論文の詳細を見る
Enhanced adhesion and migration of osteoblastic cells on titanium (Ti) surface is believed to increase the success rate of implant therapy. A GRGDSP peptide derived from fibronectin was coated on Ti surfaces using a tresyl chloride activation technique, and then MC3T3-E1 osteoblastic cells were cultured on the Ti surfaces. After 15 days, total RNA was isolated from the cells and gene expression level were analyzed by Affymetrix GeneChip system. The expression levels of many genes in MC3T3-E1 cells cultured on GRGDSP-coated Ti surface were altered when compared to uncoated Ti. In particular, the elevated mRNA levels of bone sialoprotein (BSP) and osteocalcin (OC) were successfully confirmed by reverse transcription-polymerase chain reaction (RT-PCR) and real-time PCR. In light of the results obtained, GRGDSP-coated Ti presented the potential of evolving into a useful biomaterial for successful implant therapy.
著者
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PUGDEE Kamolparn
Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo
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HAYAKAWA Tohru
Department of Dental Biomaterial, Nihon University School of Dentistry at Matsudo
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ABIKO Yoshimitsu
Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo
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CHANG Wei-Jen
School of Dentistry, College of Oral Medicine, Taipei Medical University
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LEE Sheng-Yang
School of Dentistry, College of Oral Medicine, Taipei Medical University
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Lee Sheng-yang
School Of Dentistry Taipei Medical University
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Chang Wei-jen
School Of Oral Hygiene Taipei Medical University
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Hayakawa Tohru
Department Of Dental Biomaterial Nihon University School Of Dentistry At Matsudo
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Hayakawa Tohru
Nihon Univ. Graduate School Of Dentistry At Matsudo Chiba Jpn
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Hayakawa Tohru
Department Of Appilied Biology Faculty Of Textile Science Kyoto Institute Of Technology
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Yoshinari Masao
Division Of Oral Implants Research Oral Health Science Center Tokyo Dental College
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Pugdee Kamolparn
Department Of Biochemistry And Molecular Biology Nihon University School Of Dentistry At Matsudo
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Abiko Yoshimitsu
Department Of Biochemistry Nihon University School Dentistry At Matsudo
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Abiko Y
Department Of Biochemistry And Molecular Biology Nihon University School Of Dentistry At Matsudo:res
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Abiko Yoshimitsu
Department Of Biochemistry And Molecular Biochemistry Nihon University School Of Dentistry At Matsud
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YAMAMICHI Nobuyuki
Department of Biochemistry and Molecular Biology, Nihon University School of Dentistry at Matsudo
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Chang Wei-jen
School Of Dentistry College Of Oral Medicine Taipei Medical University
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Lee Sheng-yang
School Of Dentistry College Of Oral Medicine Taipei Medical University
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Yamamichi Nobuyuki
Department Of Biochemistry And Molecular Biology Nihon University School Of Dentistry At Matsudo
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Yoshinari Masao
Division Of Oral Implants Research Oral Health Science Center Tokyo Dental Collage
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